HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS		QUICK SEARCH:   [advanced] Author: Keyword(s): Year:  Vol:  Page:

This Article Full Text Full Text (PDF) Submit a response Alert me when this article is cited Alert me when eLetters are posted Alert me if a correction is posted Citation Map Services Email this article to a friend Similar articles in this journal Similar articles in ISI Web of Science Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Citing Articles Citing Articles via HighWire Citing Articles via Google Scholar Google Scholar Articles by Suárez, F. Articles by Murray, T. G. Search for Related Content PubMed PubMed Citation Articles by Suárez, F. Articles by Murray, T. G.

Paclitaxel in the Treatment of Retinal Tumors of LH beta-Tag Murine Transgenic Model of Retinoblastoma

From the Bascom Palmer Eye Institute, Department of Ophthalmology, University of Miami, Miami, Florida.

PURPOSE. The purpose of this study was to investigate tumor control efficacy of paclitaxel in the treatment of retinal tumors harbored by the LH beta-Tag murine transgenic model of retinoblastoma.

METHODS. LH beta-Tag–positive mice (n = 5) 10 weeks of age received two 20-µL subconjunctival injections delivered with a 72-hour interval to right eyes only. Paclitaxel was dissolved in 100% dimethyl sulfoxide (DMSO) and delivered at doses of 0.5 mg, 0.25 mg, 0.125 mg, 0.0625 mg, 0.0313 mg, and 0.0152 mg in a 20-µL volume. Control mice received two subconjunctival injections of DMSO or of saline solution to right eyes only or they remained untreated. Eyes were analyzed at 16 weeks of age for residual tumor burden, which was measured by gauging the cross-sectional area of largest tumor focus.

RESULTS. Linear regression analysis of tumor burden demonstrates a statistically significant (P < 0.001) dose–response relationship between paclitaxel concentration and tumor size. Transient ocular toxicities were observed after treatment, but most had subsided at the time of analysis, 6 weeks after injection. After histologic assessment, the 0.25-mg paclitaxel dose had effectively reduced tumor burden and was associated with minimal toxicity, including mild conjunctival chemosis and fibrosis, corneal epitheliopathy, and corneal edema.

CONCLUSIONS. Subconjunctival delivery of paclitaxel effectively inhibits intraocular tumor burden in the LH beta-Tag model of retinoblastoma. This treatment modality may represent a novel strategy for the clinical management of retinoblastoma.

This article has been cited by other articles:

				M. KUWATA, K. YOSHIZAWA, M. MATSUMURA, K. TAKAHASHI, and A. TSUBURA Ocular Toxicity Caused by Paclitaxel in Neonatal Sprague-Dawley Rats In Vivo, July 1, 2009; 23(4): 555 - 560. [Abstract] [Full Text] [PDF]

				G. L. Chantada, A. C. Fandino, A. M. Carcaboso, E. Lagomarsino, M. T. G. de Davila, M. R. Guitter, A. B. Rose, J. Manzitti, G. F. Bramuglia, and D. H. Abramson A Phase I Study of Periocular Topotecan in Children with Intraocular Retinoblastoma Invest. Ophthalmol. Vis. Sci., April 1, 2009; 50(4): 1492 - 1496. [Abstract] [Full Text] [PDF]