IOVS Health Education Research
HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
QUICK SEARCH:   [advanced]


     

(Investigative Ophthalmology and Visual Science. 2007;48:3677-3682.)
© 2007 by The Association for Research in Vision and Ophthalmology, Inc.
DOI:  10.1167/iovs.06-0896
This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow Submit a response
Right arrow Alert me when this article is cited
Right arrow Alert me when eLetters are posted
Right arrow Alert me if a correction is posted
Right arrow Citation Map
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in Web of Science
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrow reprints & permissions
Citing Articles
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Romano, M. R.
Right arrow Articles by Lograno, M. D.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Romano, M. R.
Right arrow Articles by Lograno, M. D.

Evidence for the Involvement of Cannabinoid CB1 Receptors in the Bimatoprost-Induced Contractions on the Human Isolated Ciliary Muscle

Maria Rosaria Romano and Marcello Diego Lograno

From the Department of Pharmacobiology, Section of Pharmacology, University of Bari, Bari, Italy.

PURPOSE. To evaluate the bimatoprost effects in the isolated human ciliary muscle and to assess how these response can be modulated by AL8810 and SR141716A.

METHODS. In a myograph system (isometric force measurement), ciliary muscles were exposed cumulatively to PGF2{alpha}, latanoprost, travoprost, bimatoprost, and anandamide (0.1 nM-10 µM). Experiments were also conducted in the presence of AL8810 (FP receptor antagonist; 100 nM) or SR141716A (CB1 receptor antagonist; 10–100 nM). Contractions were expressed as the percentage of 10 µM carbachol-induced contractions.

RESULTS. In quiescent tissues, concentration-response curves for bimatoprost, anandamide, PGF2{alpha}, latanoprost, and travoprost were constructed. Bimatoprost showed an important contractile effect on isolated human ciliary muscle strips (Emax = 125% ± 0.09%); the maximal effect was higher than that obtained with carbachol. Contractions were inhibited by SR141716A (10 and 100 nM) and AL8810 (100 nM).

CONCLUSIONS. This study showed evidence of direct interaction of bimatoprost with the contractility of the human ciliary muscle through interaction with cannabinoid CB1 receptor and prostanoid FP receptors.






HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
Copyright © 2007 by the Association for Research in Vision and Ophthalmology