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(Investigative Ophthalmology and Visual Science. 2007;48:4187-4191.)
© 2007 by The Association for Research in Vision and Ophthalmology, Inc.
DOI:  10.1167/iovs.06-0660
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p53 Expression and Apoptosis in the Lens after Ultraviolet Radiation Exposure

Marcelo Ayala,1,2 Hilja Strid,3 Ulrika Jacobsson,3 and Per G. Söderberg2

1From the Eye Department and the 3Clinic Research Centre, Örebro University Hospital, Örebro, Sweden; and 2St. Erik's Eye Hospital, Karolinska Institute, Stockholm, Sweden.

PURPOSE. To localize p53 protein and active caspase-3 in the albino rat lens and to compare p53 mRNA and active caspase-3 expression in ultraviolet radiation (UVR) 300 nm exposed lenses and their contralateral nonexposed controls.

METHODS. Ten Sprague–Dawley albino rats were unilaterally exposed to 8 kJ/m2 UVR, and the contralateral eyes were left nonexposed. In total, four exposed lenses and their respective contralateral nonexposed lenses were analyzed by immunohistochemistry to localize p53 and active caspase-3. In addition, six exposed and contralateral nonexposed lenses were analyzed by real-time RT-PCR. Quantified p53 and caspase-3 expression were compared between the in vivo UVR 300 nm exposed lenses and the contralateral nonexposed lenses.

RESULTS. All lenses exposed to UVR developed cataract. Immunohistochemistry showed that p53 and active caspase-3 were localized in the lens epithelial cells. Quantified p53 and caspase-3 expression were significantly higher in lenses exposed to UVR than in nonexposed lenses.

CONCLUSIONS. p53 and caspase-3 expression increase in lens epithelial cells after UVR exposure. In the lens, apoptosis induced by UVR may be associated with increased p53 expression.






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