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Originally published In Press as doi:10.1167/iovs.08-2054 on June 14, 2008
 (Investigative Ophthalmology and Visual Science. 2008;49:4604-4612.)
© 2008 by The Association for Research in Vision and Ophthalmology, Inc.
DOI:  10.1167/iovs.08-2054
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Effect of Bacterial Lipopolysaccharide on Ischemic Damage in the Rat Retina

Pablo J. Franco,1 Diego C. Fernandez,1,2 Pablo H. Sande,1 María I. Keller Sarmiento,1 Mónica Chianelli,1 Daniel A. Sáenz,1 and Ruth E. Rosenstein1

1From the Laboratorio de Neuroquímica Retiniana y Oftalmología Experimental, Departamento de Bioquímica Humana, Universidad de Buenos Aires, CEFyBO/CONICET (Centro de Estudios Farmacologicos y Botanicos/Consejo Nacional de Investigaciones Científicas y Técnicas), Buenos Aires, Argentina; and 2Laboratorio de Histología, Facultad de Medicina, Universidad de Morón, Buenos Aires, Argentina.

PURPOSE. The purpose of this study was to investigate whether bacterial lipopolysaccharide (LPS) induces ischemic preconditioning in the rat retina, and, if so, whether nitric oxide (NO) is involved in this process.

METHODS. Rats were intravitreously injected with different doses of LPS (0.1, 1, or 5 µg) in one eye and vehicle in the contralateral eye 24 hours before retinal ischemia induced by increasing intraocular pressure to 120 mm Hg for 40 or 60 minutes. Subsequently, 7 or 14 days after ischemia, the rats were subjected to electroretinography and histologic analysis. One group of animals received intraperitoneal injections of NOS inhibitors, N-nitro-L-arginine methyl ester (L-NAME) aminoguanidine or N-(3-(aminomethyl)benzyl)acetamidine (W1400) before the injection of LPS or vehicle. Retinal nitric oxide synthase (NOS) activity was assessed through the conversion of 3H-L-arginine to 3H-L-citrulline.

RESULTS. One microgram (but not 0.1 or 5 µg) LPS afforded significant morphologic and functional protection in eyes exposed to ischemia-reperfusion injury. The beneficial effect of LPS was reversed by treatment with L-NAME, aminoguanidine, or W1400. LPS (1 and 5 µg, but not 0.1 µg) significantly increased retinal NOS activity.

CONCLUSIONS. These results indicate that LPS provides retinal protection against ischemia-reperfusion injury in a dose-dependent manner, probably through an inducible NOS-dependent mechanism.






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