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Targeted Knockout of the Mouse βB2-crystallin Gene (Crybb2) Induces Age-Related Cataract

¹From the Central Laboratory, Changhai Hospital, Second Military Medical University, Shanghai, People’s Republic of China; ³Department of Biochemistry and Molecular Biology, College of Basic Medical Sciences, Second Military Medical University, Shanghai, People’s Republic of China; ⁴Department of Ophthalmology, Changhai Hospital, Second Military Medical University, Shanghai, People’s Republic of China; and ⁵Department of Pathology, Changhai Hospital, Second Military Medical University, Shanghai, People’s Republic of China

PURPOSE. The βB2-crystallin gene (Crybb2) is expressed at an increasing level in the postnatal lens cortex. It has been assumed that the gene functions as structural protein, although this has not been directly tested. Here the in vivo functions of βB2-crystallin are examined via the generation of mice with a targeted disruption of Crybb2.

METHODS. Gene targeting in embryonic stem cells was used to generate mouse lines in which Crybb2 was deleted. Gene structure and protein expression were analyzed by PCR, immunoblot, and two-dimensional gel electrophoresis. Knockout mice were screened for cataract with slit lamp biomicroscopy. Microstructure of lens was analyzed by scanning and transmission electron microscopy. The resistance of crystallins in knockout mice to heat-induced denaturation and oxidative stress was examined.

RESULTS. The lens appeared to develop normally in the first months of life. In older animals, the weight and axial diameter of the lenses of knockout mice were significantly smaller than in wild-type mice. Cataracts were formed in the posterior and anterior cortex several months after birth and cataract severity increased with age. The thermal stability of the supernatant of a lens homogenate was mildly compromised. The knockout lenses also showed decreased resistance to oxidative stress.

CONCLUSIONS. βB2-crystallin is not essential for the normal development of a transparent lens in the mouse. It plays an increasingly important role in maintaining the transparency of the lens after birth, possibly by interacting with other crystallins to increase their resistance to thermal denaturation and oxidative stress.