IOVS
HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
QUICK SEARCH:   [advanced]


     

Originally published In Press as doi:10.1167/iovs.08-2117 on July 18, 2008
 (Investigative Ophthalmology and Visual Science. 2008;49:5602-5610.)
© 2008 by The Association for Research in Vision and Ophthalmology, Inc.
DOI:  10.1167/iovs.08-2117
This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow All Versions of this Article:
iovs.08-2117v1
49/12/5602    most recent
Right arrow Submit a response
Right arrow Alert me when this article is cited
Right arrow Alert me when eLetters are posted
Right arrow Alert me if a correction is posted
Right arrow Citation Map
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrow reprints & permissions
Google Scholar
Right arrow Articles by Sancho-Pelluz, J.
Right arrow Articles by Perez, M.-T.
PubMed
Right arrow PubMed Citation
Right arrow Articles by Sancho-Pelluz, J.
Right arrow Articles by Perez, M.-T.

Sialoadhesin Expression in Intact Degenerating Retinas and Following Transplantation

Javier Sancho-Pelluz,1,2,3 Kirsten A. Wunderlich,1 Uwe Rauch,4 F. Javier Romero,2 Theo van Veen,1,3 G. Astrid Limb,5,6 Paul R. Crocker,7 and Maria-Thereza Perez1,8

1From the Department of Ophthalmology and 4Vessel Wall Biology Group, Lund University, Lund, Sweden; the 2Fundación Oftalmológica del Mediterráneo (FOM) and Universidad Cardenal Herrera-CEU, Valencia, Spain; 3Experimental Ophthalmology, University Eye Hospital, Tübingen, Germany; the Divisions of 5Pathology and 6Cell Biology, UCL Institute of Ophthalmology, London, United Kingdom; the 7Wellcome Trust Biocentre, University of Dundee, Dundee, United Kingdom; and the 8Department of Ophthalmology, University of Copenhagen, Glostrup Hospital, Glostrup, Denmark.

PURPOSE. Resident microglial cells normally do not express sialoadhesin (Sn; a sialic acid-binding receptor), whereas recruited inflammatory macrophages have been shown to do so. The expression of Sn was examined in the course of photoreceptor cell degeneration and after transplantation.

METHODS. Sn expression was analyzed in retinas of rd1 and rds mice. For transplantation studies, neonatal (P2) retinal cells derived from GFP mice were injected intraocularly in adult rd1 mice and control mice. Antibodies recognizing different Sn epitopes, CD11b, and MHC-II were used to identify activated microglial cells in intact retinas and 21 days after transplantation.

RESULTS. In rd1 mice, a few CD11b-positive cells were observed in the outer nuclear layer in the central retina at postnatal day (P)11 and in increasing numbers between P12 to P21. In rds mice, CD11b-expressing cells were found from P16 onward. No Sn-expressing cells were observed within the rd1 or rds mouse retinas at any of the ages examined (up to P150). Specific staining was observed only in cells found in the vitreous margin of the retina and in surrounding tissues (sclera, cornea, ciliary body, choroid). After transplantation to normal and rd1 mice, a variable number of Sn-positive cells were detected within the grafts, in the graft-host interface, and in the subretinal space.

CONCLUSIONS. The significant activation of microglia/macrophages observed in the various stages of degeneration in rd1 and rds mouse retinas is not accompanied by Sn expression. However, Sn-expressing cells are observed after transplantation. The occurrence of such cells could be of significance for the integration and long-term survival of retinal grafts, as the expression of Sn could facilitate other phagocytic receptors.






HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
Copyright © 2008 by the Association for Research in Vision and Ophthalmology