IOVS Biophysical Journal
HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
QUICK SEARCH:   [advanced]


     

(Investigative Ophthalmology and Visual Science. 2008;49:1979-1982.)
© 2008 by The Association for Research in Vision and Ophthalmology, Inc.
DOI:  10.1167/iovs.07-1393
This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow Submit a response
Right arrow Alert me when this article is cited
Right arrow Alert me when eLetters are posted
Right arrow Alert me if a correction is posted
Right arrow Citation Map
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in ISI Web of Science
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrow reprints & permissions
Citing Articles
Right arrow Citing Articles via ISI Web of Science (1)
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Cordeiro, C. A.
Right arrow Articles by Teixeira, A. L.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Cordeiro, C. A.
Right arrow Articles by Teixeira, A. L.

Interleukin-10 Gene Polymorphism (–1082G/A) is Associated with Toxoplasmic Retinochoroiditis

Cynthia A. Cordeiro,1 Paula R. Moreira,2 Mariana S. Andrade,1 Walderez O. Dutra,2 Wesley R. Campos,1 Fernando Oréfice,1 and Antônio L. Teixeira3

1From the Uveitis Section, Department of Ophthalmology, and the 2Departments of Morphology and 3Internal Medicine, Federal University of Minas Gerais, Belo Horizonte, Brazil.

PURPOSE. Experimental data have demonstrated a relevant role for IL-10, an anti-inflammatory cytokine, in the modulation of acute ocular toxoplasmosis. Therefore, this study was conducted to investigate the possible association between an IL10 gene polymorphism at position –1082 and toxoplasmic retinochoroiditis (TR) in humans.

METHODS. One hundred patients with diagnosed TR were recruited from the Uveitis Section, Federal University of Minas Gerais. For comparison, one hundred healthy blood donors with positive serology for toxoplasmosis and without retinal signs of previous TR were included in the study. Genomic DNA was obtained from oral swabs of individuals and amplified using polymerase chain reaction (PCR) with specific primers flanking the locus –1082 of IL10 (–1082G/A). PCR products were subjected to restriction endonuclease digestion and analyzed by polyacrylamide gel electrophoresis, to distinguish allele G and A of the IL-10 gene, allowing the detection of the polymorphism and determination of genotypes.

RESULTS. There was a significant difference in the genotype distribution between TR patients and control subjects ({chi}2 = 6.33, P = 0.04). Carriers of the IL10 –1082 A allele (AA+AG genotypes) were more often patients with TR than control subjects ({chi}2 = 5.97, P = 0.01, OR, 2.55; 95% CI, 1.11 < OR < 5.55). In a subgroup analysis, there was no significant difference in genotypes and allele carriage regarding visual acuity, involvement of both eyes and TR recurrence.

CONCLUSIONS. This study suggests that the genotypes related with a low production of IL-10 may be associated with the occurrence of TR.






HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
Copyright © 2008 by the Association for Research in Vision and Ophthalmology