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The LOXL1 Gene Variations Are Not Associated with Primary Open-Angle and Primary Angle-Closure Glaucomas

¹From the Kallam Anji Reddy Molecular Genetics Laboratory and the ²VST Centre for Glaucoma Care, L. V. Prasad Eye Institute, Hyderabad, India; and the ³Queensland Eye Institute, Brisbane, Queensland, Australia.

PURPOSE. Glaucoma is a complex disease involving multiple genetic factors. Recently, single nucleotide polymorphisms (SNPs) in the LOXL1 gene have been implicated in exfoliation syndrome (XFS) and exfoliation glaucoma (XFG) but not in the primary glaucomas. This study was conducted to determine the possible involvement of these SNPs in cases of primary open-angle glaucoma (POAG) and primary angle-closure glaucoma (PACG).

METHODS. The three associated SNPs of LOXL1 (rs1048661, rs3825942, and rs2165241) were screened in 208 unrelated and clinically well-characterized glaucoma cases comprising patients with POAG (n = 112) or PACG (n = 96) along with 105 ethnically matched normal control subjects from Indian populations. Subjects with exfoliative material on the lens and radial pigmentation in the periphery of the lens that could be earlier signs of XFS were excluded. These SNPs were screened by resequencing and further confirmed by PCR-based restriction digestions. Haplotypes were generated with the three SNPs in cases and control subjects, and linkage disequilibrium (LD) and haplotype analysis were performed with the Haploview software, which uses the EM (expectation-maximization) algorithm.

RESULTS. The SNPs of LOXL1 did not exhibit any significant association with POAG or PACG, unlike previous studies from Icelandic, Swedish, U.S., and Australian populations with XFS/XFG. Haplotypes generated with these intragenic SNPs did not indicate any significant risk with POAG or PACG phenotypes. The risk haplotype G-G in XFS/XFG in other populations was present in 46% of the normal control subjects in the present cohort.

CONCLUSIONS. The results from the present study do not indicate the involvement of the LOXL1 SNPs in POAG and PACG.

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				Y. Liu, S. Schmidt, X. Qin, J. Gibson, K. Hutchins, C. Santiago-Turla, J. L. Wiggs, D. L. Budenz, S. Akafo, P. Challa, et al. Lack of Association between LOXL1 Variants and Primary Open-Angle Glaucoma in Three Different Populations Invest. Ophthalmol. Vis. Sci., August 1, 2008; 49(8): 3465 - 3468. [Abstract] [Full Text] [PDF]