IOVS
HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
QUICK SEARCH:   [advanced]


     

(Investigative Ophthalmology and Visual Science. 2008;49:3305-3311.)
© 2008 by The Association for Research in Vision and Ophthalmology, Inc.
doi:10.1167/iovs.07-0456
This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow Submit a response
Right arrow Alert me when this article is cited
Right arrow Alert me when eLetters are posted
Right arrow Alert me if a correction is posted
Right arrow Citation Map
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in Web of Science
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrow reprints & permissions
Citing Articles
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Sameermahmood, Z.
Right arrow Articles by Rema, M.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Sameermahmood, Z.
Right arrow Articles by Rema, M.

Curcumin Modulates SDF-1{alpha}/CXCR4–Induced Migration of Human Retinal Endothelial Cells (HRECs)

Zaheer Sameermahmood,1 Muthuswamy Balasubramanyam,1 Thangavel Saravanan,1 and Mohan Rema2

1From the Departments of Cell and Molecular Biology and 2Ophthalmology, Madras Diabetes Research Foundation, Chennai, India.

PURPOSE. The stromal-derived factor (SDF)-1{alpha} and the CXC receptor (CXCR)-4 jointly regulate the trafficking of various cell types and play a pivotal role in cell migration, proliferation, and survival. The purpose of this study was to assess whether curcumin inhibits human retinal endothelial cell (HREC) migration by interfering with SDF-1{alpha}/CXCR4 signaling.

METHODS. Primary HREC culture was established and maintained in endothelial growth medium. The viability and proliferation of HRECs were assessed by MTT and thymidine uptake assays, respectively. The effect of SDF-1{alpha}–induced HREC migration (chemotaxis) in the presence and absence of curcumin was determined using the Boyden chamber migration assay. Intracellular Ca2+ concentration was measured by fluorometric analysis. Immunofluorescence and Western blot analyses were performed to quantify CXCR4, phosphorylated AKT, and PI3-kinase expression levels.

RESULTS. HREC migration increased in a dose-dependent manner (1, 10, 50, and 100 ng/mL; P < 0.001) in SDF-1{alpha}–treated cells. In contrast, AMD3100, an inhibitor of CXCR4 effectively inhibited HREC migration dose dependently. HREC migration was decreased when the cells were exposed to EGTA, a chelator of Ca2+. Curcumin also blocked Ca2+ influx, an important signal for HREC migration. In addition, curcumin significantly (P < 0.001) decreased SDF-1{alpha}–induced HRECs migration and downregulated SDF-1{alpha}–induced expression of CXCR4, phospho-AKT, phospho-phosphatidylinositol-3-kinase (PI3-K), and eNOS.

CONCLUSIONS. This study indicates that curcumin has an inhibitory effect on SDF-1{alpha}–induced HREC migration. The plausible mechanism of action could be upstream blockage of Ca2+ influx and the downstream reduction of PI3-K/AKT signals.






HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
Copyright © 2008 by the Association for Research in Vision and Ophthalmology