IOVS
HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
QUICK SEARCH:   [advanced]


     

Originally published In Press as doi:10.1167/iovs.07-1383 on April 25, 2008
 (Investigative Ophthalmology and Visual Science. 2008;49:3611-3616.)
© 2008 by The Association for Research in Vision and Ophthalmology, Inc.
doi:10.1167/iovs.07-1383
This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow All Versions of this Article:
iovs.07-1383v1
49/8/3611    most recent
Right arrow Submit a response
Right arrow Alert me when this article is cited
Right arrow Alert me when eLetters are posted
Right arrow Alert me if a correction is posted
Right arrow Citation Map
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in Web of Science
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrow reprints & permissions
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Web of Science (5)
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Churchill, A. J.
Right arrow Articles by Ray, D. W.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Churchill, A. J.
Right arrow Articles by Ray, D. W.

VEGF Polymorphisms Are Associated with Severity of Diabetic Retinopathy

Amanda J. Churchill,1 James G. Carter,1 Conor Ramsden,1 Steven J. Turner,1 Anna Yeung,1 Paul E. C. Brenchley,2 and David W. Ray2

1From the Unit of Ophthalmology, Bristol Eye Hospital, University of Bristol, Bristol, United Kingdom; and the 2School of Clinical and Laboratory Medicine, University of Manchester, Manchester, United Kingdom.

PURPOSE. To determine whether single nucleotide polymorphisms (SNPs) in the vascular endothelial growth factor (VEGF) gene are associated with severity of diabetic retinopathy.

METHODS. A case–control study was conducted in which 45 individuals with type 1 or 2 diabetes with proliferative diabetic retinopathy (PDR) and 61 individuals with type 1 or 2 diabetes without retinopathy (DWR) were genotyped for 14 SNPs in the VEGF promoter and gene.

RESULTS. Three of the promoter SNP genotypes, –160C, –152A (rs13207351), and –116A (rs1570360), showed significant independent associations with PDR, as well as the minihaplotype CAA (P = 0.00017). Two promoter haplotypes were associated with severity of retinopathy: –460C, –417T, –172C, –165C, –160C, –152A, –141A, –116A, +405C was associated with PDR (OR [95% CI] = 29.92 [3.91, 228.78], P = 1.62 x 10–5) and –460C, –2417T, –172C, –165C, –160C, –152A, –141A, –116G, +405G was associated with DWR (OR = 0.05 [0.01, 0.35], P = 0.000373). Furthermore, two haplotype-tagged (ht) SNPs, +4618 (rs735286) and +5092 (rs2146323), and five htSNP haplotypes were associated with severity of retinopathy. When the nine promoter/5' untranslated region [UTR] and five htSNP genotypes were combined into a 14-SNP haplotype, a single haplotype, –460C, –417T, –172C, –165C, –160C, –152A, –141A, –116A, +405C, +674T, +4618C, +5092A, +9162C, +9512C was found to be significantly associated with the PDR group (OR = 18.45 [2.35, 144.67], P = 0.00622).

CONCLUSIONS. A clear association was demonstrated between VEGF SNPs and severity of diabetic retinopathy. Furthermore, two of the htSNP haplotypes appear to be more generalized markers for angiogenesis, in that these have been found in prior work to be associated with neovascular age-related macular degeneration.




This article has been cited by other articles:


Home page
 J. Epidemiol. Community HealthHome page
C Ren, A Baccarelli, E Wilker, H Suh, D Sparrow, P Vokonas, R Wright, and J Schwartz
Lipid and endothelium-related genes, ambient particulate matter, and heart rate variability--the VA Normative Aging Study
J Epidemiol Community Health, January 1, 2010; 64(01): 49 - 56.
[Abstract] [Full Text] [PDF]

Home page
 IOVSHome page
S. Abhary, K. P. Burdon, A. Gupta, S. Lake, D. Selva, N. Petrovsky, and J. E. Craig
Common Sequence Variation in the VEGFA Gene Predicts Risk of Diabetic Retinopathy
Invest. Ophthalmol. Vis. Sci., December 1, 2009; 50(12): 5552 - 5558.
[Abstract] [Full Text] [PDF]

Home page
 Hum Mol GenetHome page
R. Zarbock, D. Hendig, C. Szliska, K. Kleesiek, and C. Gotting
Vascular endothelial growth factor gene polymorphisms as prognostic markers for ocular manifestations in pseudoxanthoma elasticum
Hum. Mol. Genet., September 1, 2009; 18(17): 3344 - 3351.
[Abstract] [Full Text] [PDF]

Home page
 Arch OphthalmolHome page
M. S. Roy, D. M. Hallman, Y.-P. Fu, M. Machado, and C. L. Hanis
Assessment of 193 Candidate Genes for Retinopathy in African Americans With Type 1 Diabetes
Arch Ophthalmol, May 1, 2009; 127(5): 605 - 612.
[Abstract] [Full Text] [PDF]


HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
Copyright © 2008 by the Association for Research in Vision and Ophthalmology