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Originally published In Press as doi:10.1167/iovs.08-1915 on May 9, 2008
 (Investigative Ophthalmology and Visual Science. 2008;49:3961-3969.)
© 2008 by The Association for Research in Vision and Ophthalmology, Inc.
DOI:  10.1167/iovs.08-1915
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Cultured Porcine Trabecular Meshwork Cells Display Altered Lysosomal Function When Subjected to Chronic Oxidative Stress

Paloma B. Liton, Yizhi Lin, Coralia Luna, Guorong Li, Pedro Gonzalez, and David L. Epstein

From the Department of Ophthalmology, Duke University Eye Center, Durham, North Carolina.

PURPOSE. To investigate the effects of chronic oxidative stress on lysosomal function in trabecular meshwork (TM) cells.

METHODS. Confluent cultures of porcine TM cells were grown for 2 weeks in physiological (5% O2) or hyperoxic conditions (40% O2) in the presence or absence of the protease inhibitor leupeptin (10 µM). The following parameters were quantified using the fluorogenic probes indicated within parentheses: autofluorescence, intracellular reactive oxygen species (ROS; H2DCFDA), mitochondrial membrane potential (JC-1), mitochondrial content (Mitotracker Red; Invitrogen-Molecular Probes, Eugene, OR), lysosomal content (acridine orange and Lysotracker Red [Invitrogen-Molecular Probes]), autophagic vacuole content (MDC), SA-β-galactosidase (FDG), and cathepsin activities (z-FR-AMC). Cathepsin levels were quantified by qPCR and Western blot analysis. Ultrastructural analysis was performed by transmission electron microscopy.

RESULTS. Prolonged exposure of porcine TM cells to a hyperoxic environment led to an increase in ROS production and oxidized material. Electron micrographs revealed the cytoplasmic accumulation of lipofuscin-loaded lysosomes. Augmented lysosomal and autophagic vacuole content was confirmed with specific fluorophores. The mRNA and protein levels of several cathepsins were upregulated with oxidative stress. This upregulated expression did not correlate with increased lysosomal activity.

CONCLUSIONS. The results indicate that chronic exposure of TM cells to oxidative stress causes the accumulation of nondegradable material within the lysosomal compartment, leading to diminished lysosomal activity. Since the lysosomal system is responsible for the continuous turnover of cellular organelles, impaired lysosomal activity may lead to progressive failure of cellular TM function with age.






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