IOVS Clinical and Diagnostic Laboratory Immunology
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Originally published In Press as doi:10.1167/iovs.07-1456 on April 30, 2008
 (Investigative Ophthalmology and Visual Science. 2008;49:4089-4095.)
© 2008 by The Association for Research in Vision and Ophthalmology, Inc.
DOI:  10.1167/iovs.07-1456
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Collagen Distribution in the Human Vitreoretinal Interface

Theodorus L. Ponsioen,1,2 Marja J. A. van Luyn,2 Roelofje J. van der Worp,1,2 Jan C. van Meurs,3 Johanna M. M. Hooymans,1 and Leonoor I. Los1

1From the Departments of Ophthalmology and 2Pathology and Medical Biology, University Medical Center Groningen, University of Groningen, Groningen, The Netherlands; and the 3The Rotterdam Eye Hospital and Erasmus Medical Center Rotterdam, Rotterdam, The Netherlands.

PURPOSE. To evaluate the presence of collagen types I to VII, IX, XI, and XVIII at the posterior pole, the equator and the pre-equatorial area in human donor eyes, since collagens are important macromolecules that contribute to vitreoretinal adhesion at the vitreoretinal interface.

METHODS. Freshly isolated human retinectomy samples from the equator were used for reverse transcription–polymerase chain reaction to detect mRNA of the above-mentioned collagens. In addition, human donor eyes and equatorial retinectomy samples were embedded in paraffin, stained with antibodies against the collagens and evaluated by light microscopy (LM).

RESULTS. Retinectomy samples expressed mRNA of all tested collagen types. By LM, vitreous cortex was positive for collagen types II, V, IX, and XI. In all three regions within the donor eyes and in the retinectomy samples, the internal limiting membrane (ILM) showed types IV, VI, and XVIII; the retinal vasculature was positive for types I to VI and XVIII in most specimens; and the retinal layers showed condensed spots of type VII. In addition, type VII increased in density and in distribution over the retinal layers toward the posterior pole.

CONCLUSIONS. Staining patterns of collagen types I to V, IX, XI, and XVIII confirmed previous observations. Important new findings include the presence of type VI in the ILM and type VII in several layers of the retina. Both collagens can anchor matrix components, and type VI could be involved in vitreoretinal attachment. Furthermore, the presence of collagen mRNA in human retinectomy samples may be an indication of postnatal collagen production by retinal cells.






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