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Originally published In Press as doi:10.1167/iovs.07-1380 on May 9, 2008
 (Investigative Ophthalmology and Visual Science. 2008;49:4145-4153.)
© 2008 by The Association for Research in Vision and Ophthalmology, Inc.
DOI:  10.1167/iovs.07-1380
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Reprogramming Progeny Cells of Embryonic RPE to Produce Photoreceptors: Development of Advanced Photoreceptor Traits under the Induction of neuroD

Lina Liang,1 Run-Tao Yan,1 Xiumei Li,1,2 Melissa Chimento,1 and Shu-Zhen Wang1

1From the Department of Ophthalmology, University of Alabama at Birmingham, Birmingham, Alabama; and the 2Zhongshan Ophthalmic Center, Sun Yat-sen University, Guangzhou, China.

PURPOSE. In examining the prospect of producing functional photoreceptors by reprogramming the differentiation of RPE progeny cells, this study was conducted to investigate whether reprogrammed cells can develop highly specialized ultrastructural and physiological traits that characterize retinal photoreceptors.

METHODS. Cultured chick RPE cells were reprogrammed to differentiate along the photoreceptor pathway by ectopic expression of neuroD. Cellular ultrastructure was examined with electron microscopy. Cellular physiology was studied by monitoring cellular free calcium (Ca2+) levels in dark-adapted cells in response to light and in light-bleached cells in response to 9-cis-retinal.

RESULTS. Reprogrammed cells were found to localize red opsin protein appropriately to the apex. These cells developed inner segments rich in mitochondria, and while in culture, some formed rudimentary outer segments, analogous to those of developing photoreceptors in the retina. In response to light, reprogrammed cells reduced their Ca2+ levels, as observed with developing retinal photoreceptors in culture. Further, on exposure to 9-cis-retinal, the light-bleached, reprogrammed cells increased their Ca2+ levels, reminiscent of visual cycle recovery.

CONCLUSIONS. These results indicate the potential of reprogrammed cells to develop advanced ultrastructural and physiological traits of photoreceptors and point to reprogramming progeny cells of embryonic RPE as a possible alternative in producing developing photoreceptors.




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