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Detection of Optic Nerve Head Neural Canal Opening within Histomorphometric and Spectral Domain Optical Coherence Tomography Data Sets

¹From the Optic Nerve Head Research Laboratory, ³Electrodiagnostic Services, and the ⁴Ocular Biomechanics Laboratory, Devers Eye Institute, Legacy Health System, Portland, Oregon; and the ²Biomedical Engineering Department, Tulane University, New Orleans, Louisiana.

PURPOSE. To assess the ability to detect the neural canal opening (NCO) and its characteristics within three-dimensional (3-D) histomorphometric and 3-D spectral domain optical coherence tomography (SD-OCT) reconstructions of the optic nerve head from nonhuman primate (NHP) eyes.

METHODS. NCO was delineated within 40 radial, sagittal sections of 3-D histomorphometric reconstructions of 44 normal eyes of 38 NHPs, each perfusion fixed at IOP 10 mm Hg, and 3-D SD-OCT (Spectralis; Heidelberg Engineering, Heidelberg, Germany) volumes acquired in vivo from a separate group of 33 normal eyes of 24 NHPs. Within all reconstructions, a least-squares ellipse was fitted to the 80 NCO points. For each eye, the dimensions and plane error (a gauge of planarity) of the fitted ellipse were calculated.

RESULTS. The NCO was successfully delineated within every section of each histomorphometric and SD-OCT reconstruction. Median plane error was similar within histomorphometric and SD-OCT volumes (8 µm, range 4–19, histomorphometry, and 10 µm, range 4–26, SD-OCT) and was small relative to the size of the ellipse. Median histomorphometric ellipse dimensions were 1453 µm (major axis, range 1218–1737) and 1066 µm (minor axis, range 808–1263). Median SD-OCT ellipse dimensions were 1512 µm (major axis, range 1191–1865) and 1060 µm (minor axis, range 772–1248).

CONCLUSIONS. The NCO is biologically continuous and relatively planar within all 3-D histomorphometric and SD-OCT reconstructions. These characteristics support its further evaluation as a reference plane for cross-sectional and longitudinal measurement of optic nerve head structures using 3-D SD-OCT.

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				N. G. Strouthidis, H. Yang, J. C. Downs, and C. F. Burgoyne Comparison of Clinical and Three-Dimensional Histomorphometric Optic Disc Margin Anatomy Invest. Ophthalmol. Vis. Sci., May 1, 2009; 50(5): 2165 - 2174. [Abstract] [Full Text] [PDF]