IOVS Heart
HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
QUICK SEARCH:   [advanced]


     

Originally published In Press as doi:10.1167/iovs.07-1197 on August 15, 2008
 (Investigative Ophthalmology and Visual Science. 2009;50:414-423.)
© 2009 by The Association for Research in Vision and Ophthalmology, Inc.
DOI:  10.1167/iovs.07-1197
This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow All Versions of this Article:
iovs.07-1197v1
50/1/414    most recent
Right arrow Submit a response
Right arrow Alert me when this article is cited
Right arrow Alert me when eLetters are posted
Right arrow Alert me if a correction is posted
Right arrow Citation Map
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrow reprints & permissions
Citing Articles
Right arrow Citing Articles via ISI Web of Science (1)
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Abbott, C. J.
Right arrow Articles by Pianta, M. J.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Abbott, C. J.
Right arrow Articles by Pianta, M. J.

Relationship of the Optical Coherence Tomography Signal to Underlying Retinal Histology in the Tree Shrew (Tupaia belangeri)

Carla J. Abbott,1 Neville A. McBrien,1 Ulrike Grünert,1,2 and Michael J. Pianta1

1From the Department of Optometry and Vision Sciences, The University of Melbourne, Parkville, Victoria, Australia; and the 2National Vision Research Institute of Australia, Carlton, Victoria, Australia.

PURPOSE. To interpret the retinal origin of the optical coherence tomography (OCT) signal by objectively (i.e., minimal investigator bias) aligning in vivo OCT longitudinal reflectivity profiles (LRPs) with corresponding vertical histologic sections.

METHODS. The Zeiss StratusOCT system was used to obtain retinal B-scans in vivo in eyes from adult tree shrews. Subsequently, the retinas were fixed and embedded. Semithin vertical sections through the retina were obtained from the same locations as the LRPs. A statistical correlation procedure that accounted for axial tissue shrinkage determined the best relationship between features in the LRP and sublaminae boundaries in corresponding histology sections.

RESULTS. For the optimal relationship, the three regions of high reflectivity in the inner OCT signal corresponded to (1) the nerve fiber and ganglion cell layers, (2) the inner plexiform layer and amacrine cell somas, and (3) the outer plexiform layer. The two regions of low reflectivity in the inner OCT signal corresponded to (1) the somas of Müller, bipolar, and horizontal cells in the inner nuclear layer and (2) the outer nuclear layer. The outer OCT signal had a region of high reflectivity that corresponded to the photoreceptor inner and outer segments, the pigment epithelium, Bruch’s membrane, and at least part of the choriocapillaris.

CONCLUSIONS. These results provide a clear interpretation for the OCT signal in terms of the underlying retinal anatomy. This interpretation can be used in vivo to identify sublaminae affected by retinal disease and has implications for the origin of the inner OCT signal in human retina.






HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
Copyright © 2009 by the Association for Research in Vision and Ophthalmology