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Originally published In Press as doi:10.1167/iovs.08-2877 on June 3, 2009
 (Investigative Ophthalmology and Visual Science. 2009;50:5472-5478.)
© 2009 by The Association for Research in Vision and Ophthalmology, Inc.
doi:10.1167/iovs.08-2877
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Thrombospondin-1–Mediated Regulation of Microglia Activation after Retinal Injury

Tat Fong Ng, Bruce Turpie, and Sharmila Masli

From the Schepens Eye Research Institute, Department of Ophthalmology, Harvard Medical School, Boston, Massachusetts.

Corresponding author: Sharmila Masli, Schepens Eye Research Institute, 20 Staniford Street, Boston, MA 02114; sharmila.masli{at}schepens.harvard.edu.

Purpose. Thrombospondin (TSP)-1 has been demonstrated to play a vital role in immune privilege. The functional phenotype of ocular antigen-presenting cells that contributes to the immune privilege status of the eye is dependent on their expression of TSP-1. Microglia, the local antigen-presenting cells in the retina, undergo rapid activation in response to injury and have the ability to produce both proinflammatory and regenerative neurotrophic factors. In this study, the authors examined TSP-1 as a potential regulator of these phenotype of microglia activated in response to retinal injury.

Methods. Expression of markers associated with activated microglia were examined by immunofluorescent staining and semiquantitative real-time PCR analysis of retina derived from WT or TSP-1 null mice at various time intervals after light- or laser-induced retinal injury.

Results. In the absence of TSP-1, microglia in uninjured retina express major histocompatibility complex class II and migrate to the outer layers of the retina. Constitutively increased expression of activated microglia-derived inflammatory molecules such as TNF-{alpha} and iNOS is detectable in TSP-1 null retina compared with WT controls. After both light-induced and laser-induced retinal injury, enhanced migration of microglia is detected in TSP-1 null retina, and these microglia express markers associated with a proinflammatory phenotype. Compared with WT retina, TSP-1 null retina fails to recover from the laser-induced injury, resulting in irreversible damage.

Conclusions. TSP-1 supports an anti-inflammatory phenotype of microglia in the retina and promotes recovery from injury.






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