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Originally published In Press as doi:10.1167/iovs.08-2493 on November 7, 2008
 (Investigative Ophthalmology and Visual Science. 2009;50:1433-1439.)
© 2009 by The Association for Research in Vision and Ophthalmology, Inc.
doi:10.1167/iovs.08-2493
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Neuroprotective Effect of an Antioxidant, Lutein, during Retinal Inflammation

Mariko Sasaki,1,2,3 Yoko Ozawa,1,2,3 Toshihide Kurihara,1,2 Kousuke Noda,1,2 Yutaka Imamura,1,2 Saori Kobayashi,4 Susumu Ishida,1,2 and Kazuo Tsubota2

1From the Laboratory of Retinal Cell Biology and the 2Department of Ophthalmology, Keio University School of Medicine, Tokyo, Japan; and 4Wakasa Seikatsu Co., Ltd., Kyoto, Japan.

PURPOSE. Lutein has been the focus of recent study as a possible therapeutic approach for retinal diseases, but the molecular mechanism of its neuroprotective effect remains to be elucidated. The aim of this study was to investigate, with the use of a mouse endotoxin-induced uveitis (EIU) model, the neuroprotective effects of lutein against retinal neural damage caused by inflammation.

METHODS. EIU was induced by intraperitoneal injection of lipopolysaccharide (LPS). Each animal was given a subcutaneous injection of lutein or vehicle three times: concurrently with and 3 hours before and after the LPS injection. Analysis was carried out 24 hours after EIU induction. Levels of rhodopsin protein and STAT3 activation were analyzed by immunoblotting. Lengths of the outer segments of the photoreceptor cells were measured. Dark-adapted full-field electroretinograms were recorded. Oxidative stress in the retina was analyzed by dihydroethidium and fluorescent probe. Expression of glial fibrillary acidic protein (GFAP) was shown immunohistochemically.

RESULTS. The EIU-induced decrease in rhodopsin expression followed by shortening of the outer segments and reduction in a-wave amplitude were prevented by lutein treatment. Levels of STAT3 activation, downstream of inflammatory cytokine signals, and reactive oxygen species (ROS), which are both upregulated during EIU, were reduced by lutein. Pathologic change of Müller glial cells, represented by GFAP expression, was also prevented by lutein.

CONCLUSIONS. The present data revealed that the antioxidant lutein was neuroprotective during EIU, suggesting a potential approach for suppressing retinal neural damage during inflammation.






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