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Originally published In Press as doi:10.1167/iovs.08-1900 on September 20, 2008
 (Investigative Ophthalmology and Visual Science. 2009;50:1575-1579.)
© 2009 by The Association for Research in Vision and Ophthalmology, Inc.
doi:10.1167/iovs.08-1900
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Circulating Hematopoietic Stem Cells in Patients with Idiopathic Choroidal Neovascularization

Manabu Sasahara, Atsushi Otani, Yuko Yodoi, and Nagahisa Yoshimura

From the Department of Ophthalmology and Visual Sciences, Kyoto University Graduate School of Medicine, Kyoto, Japan.

PURPOSE. In a prior study, the correlation was investigated between circulating hematopoietic stem cells (HSCs) and the activity or severity of choroidal neovascularization (CNV) in patients with age-related macular degeneration (AMD). The present study was designed to explore the role of circulating HSCs in the pathogenesis of CNV in patients with idiopathic (i)CNV.

METHODS. Thirteen patients with clinically documented iCNV and 10 age–sex matched patients without iCNV or systemic disease were enrolled in a case–control study. Circulating HSCs were collected from the patients’ peripheral blood and cultured. Colony-forming capacity, migration activity, and invasion activity of HSCs were experimentally analyzed.

RESULTS. Colony-forming units by Hill’s assay (CFU-Hill) were markedly lower (P < 0.001) in patients with iCNV (12.8 ± 3.2) than in healthy patients (67.6 ± 12.6). Invasion activities of HSCs were reduced significantly in patients with iCNV. CFU-Hill was impaired in the patients (4.0 ± 1.2) with CNV more than 1500 µm compared with the patients (20.3 ± 4.0) with CNV less than 1500 µm (P = 0.006).

CONCLUSIONS. Similar to the previous finding of CNV associated with AMD, impaired functional activities of circulating HSCs were observed in patients with iCNV, which correlated with the size of iCNV. These results in young patients provide evidence to support the role of circulating HSCs in the pathogenesis of CNV.






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