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Originally published In Press as doi:10.1167/iovs.08-2659 on December 5, 2008
 (Investigative Ophthalmology and Visual Science. 2009;50:1948-1955.)
© 2009 by The Association for Research in Vision and Ophthalmology, Inc.
doi:10.1167/iovs.08-2659
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Selective Hcn1 Channels Inhibition by Ivabradine in Mouse Rod Photoreceptors

Gian Carlo Demontis,1 Claudia Gargini,1 Timoteo Giacomo Paoli,1 and Luigi Cervetto2

1From the Dipartimento di Psichiatria, Neurobiologia, Farmacologia e Biotecnologie and the 2Dipartimento di Fisiologia Umana, Università di Pisa, Pisa, Italy.

PURPOSE. To evaluate in mammalian rod photoreceptors the selectivity for hyperpolarization-activated cyclic nucleotide-gated (Hcn1, coded by Hcn1) over potassium-selective (Kir 2.4, coded by Kcnj14) channels of ivabradine, a selective inhibitor of the cardiac "funny" current (If).

METHODS. Rods were isolated from the mouse retina and voltage clamped by the perforated-patch technique. The hyperpolarization-activated current (Ih) was blocked by ivabradine during repetitive stimulation with activating/deactivating voltage steps from –80 to –30 mV, from a holding of –35 mV.

RESULTS. Full inhibition was observed at a high concentration of ivabradine (30 µM), with intermediate effects at 3 and 0.3 µM. Steady state activation and activation kinetics of the ivabradine- and CsCl-blocked currents were similar, consistent with the block by ivabradine of ion permeation through Hcn1 channels. Hcn1 blockade was also consistent with the lack of current reactivation during long steps at –110 mV. At doses that fully block Ih, ivabradine does not affect the inward rectifier current through potassium-selective Kir 2.4 channels or the outward currents evoked by stepping up from –80 to 50 mV.

CONCLUSIONS. In mammalian rods, ivabradine is a selective inhibitor of Hcn1 channels. Phosphenes perception in response to abrupt changes in luminance, which has been transiently reported in a dose-dependent way by few patients treated with ivabradine, was consistent with Hcn1 inhibition in rods.




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