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Originally published In Press as doi:10.1167/iovs.08-1949 on December 5, 2008
 (Investigative Ophthalmology and Visual Science. 2009;50:2245-2254.)
© 2009 by The Association for Research in Vision and Ophthalmology, Inc.
doi:10.1167/iovs.08-1949
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A New Model of Experimental Autoimmune Keratoconjunctivitis Sicca (KCS) Induced in Lewis Rat by the Autoantigen Klk1b22

Guomin Jiang,1,2 Yan Ke,1,2 Deming Sun,1 Hao Li,1 Mark Ihnen,1 Marcia M. Jumblatt,1 Gary Foulks,1 Yali Wang,1 Yang Bian,1 Henry J. Kaplan,1 and Hui Shao1

1From the Department of Ophthalmology and Visual Sciences, Kentucky Lions Eye Center, School of Medicine, University of Louisville, Louisville, Kentucky.

PURPOSE. This study was designed to generate an inducible autoimmune model of keratoconjunctivitis sicca (KCS) for study of pathogenesis of the disease.

METHODS. Lewis rats were immunized with a mixture of lacrimal and salivary gland extract or recombinant mouse protein kallikrein 1b22 (Klk1b22) emulsified in complete Freund’s adjuvant (CFA). For disease induction by adoptive transfer of primed cells, donor rats were received with T-cell blasts. KCS were observed by either clinical signs or histology.

RESULTS. The autoantigen Klk1b22, isolated from the lacrimal and salivary glands, readily induced Sjögren’s syndrome (SS)-like KCS in the recipients. The diseased animals presented the clinical and pathologic symptoms that resemble related human disease. Most immunized rats showed an increase, then a decrease in tear volume, together with corneal opacity and ocular lesions. Histologic examination revealed that the rats displayed the cardinal signs of primary SS-like KCS, including marked lymphocytic infiltration of the lacrimal and salivary glands and destruction of the acinar cells. Immunofluorescence studies showed that both CD8+ and CD4+ T cells were heavily infiltrated, with the former cells predominant in the damaged ducts. Finally, adoptive transfer of Klk1b22-reactive T cells induced more severe disease with earlier onset.

CONCLUSIONS. Klk1b22 is an autoantigen for inducing an experimental SS-like KCS in Lewis rats. The availability of this new and reproducible rat model should provide a new and needed tool for studying the pathogenesis of SS.






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