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Originally published In Press as doi:10.1167/iovs.08-2542 on January 31, 2009
 (Investigative Ophthalmology and Visual Science. 2009;50:2989-2993.)
© 2009 by The Association for Research in Vision and Ophthalmology, Inc.
doi:10.1167/iovs.08-2542
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In Vivo Imaging of the Mouse Model of X-Linked Juvenile Retinoschisis with Fourier Domain Optical Coherence Tomography

Jing Xu,1 Laurie L. Molday,2 Robert S. Molday,2 and Marinko V. Sarunic1

1From the School of Engineering Science, Simon Fraser University, Burnaby, British Columbia, Canada; and the 2Department of Biochemistry and Molecular Biology, Centre for Macular Research, University of British Columbia, Vancouver, British Columbia, Canada.

PURPOSE. The purpose of this study was to investigate Fourier domain optical coherence tomography (FD OCT) as a noninvasive tool for retinal imaging in the Rs1h-knockout mouse (model for X-linked juvenile retinoschisis).

METHODS. A prototype spectrometer-based FD OCT system was used in combination with a custom optical beam–scanning platform. Images of the retinas from wild-type and Rs1h-knockout mice were acquired noninvasively with FD OCT with the specimen anesthetized. At the completion of the noninvasive FD OCT imaging, invasive retinal cross-sectional images (histology) were acquired from a nearby region for comparison to the FD OCT images.

RESULTS. The retinal layers were identifiable in the FD OCT images, permitting delineation and thickness measurement of the outer nuclear layer (ONL). During FD OCT in vivo imaging of the Rs1h-knockout mouse, holes were observed in the inner nuclear layer (INL), and retinal cell disorganization was observed as a change in the backscattering intensity profile. Comparison of the ONL measurements acquired noninvasively with FD OCT to measurements taken using histology at nearby locations showed a degeneration of roughly 30% of the ONL by the age of 2 months in Rs1h-knockout mice relative to wild-type.

CONCLUSIONS. FD OCT was demonstrated to be effective for noninvasive imaging of retinal degeneration and observation of retinal holes in Rs1h-knockout mice.




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D. C. Lee, J. Xu, M. V. Sarunic, and O. L. Moritz
Fourier Domain Optical Coherence Tomography as a Noninvasive Means for In Vivo Detection of Retinal Degeneration in Xenopus laevis Tadpoles
Invest. Ophthalmol. Vis. Sci., February 1, 2010; 51(2): 1066 - 1070.
[Abstract] [Full Text] [PDF]


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