HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS		QUICK SEARCH:   [advanced] Author: Keyword(s): Year:  Vol:  Page:

This Article Full Text Full Text (PDF) All Versions of this Article: iovs.08-2448v1 50/8/3907    most recent Submit a response Alert me when this article is cited Alert me when eLetters are posted Alert me if a correction is posted Citation Map Services Email this article to a friend Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Citing Articles Citing Articles via Google Scholar Google Scholar Articles by Smith, R. T. Articles by Laine, A. Search for Related Content PubMed PubMed Citation Articles by Smith, R. T. Articles by Laine, A.

Lipofuscin and Autofluorescence Metrics in Progressive STGD

¹From the Departments of Ophthalmology and ²Biomedical Engineering, Columbia University, New York, New York.

PURPOSE. To evaluate Stargardt disease (STGD) progression and relative lipofuscin levels via autofluorescence image analysis.

METHODS. The relationship between focally increased autofluorescence (FIAF), geographic atrophy (GA) and focally decreased autofluorescence (FDAF) was analyzed in serial, registered autofluorescence (AF) scans of 10 patients with STGD (20 eyes, 40 scans; mean follow-up, 2.0 years) using automated techniques.

RESULTS. GA progressed uniformly in a transition zone with minimal FIAF. Only 4.3% of FIAF progressed to GA or FDAF, despite significant progression of GA (median 30%/year) and FDAF (mean, 29%/year). As a spatial predictor, the mean chance of FIAF for progression to FDAF was 4.3% ± 4.4%, significantly less than that of random areas (6.7% ± 4.0%, P = 0.029, Mann-Whitney test). In the seven eyes with GA, the mean chance of FIAF in the transition zone for transition to GA was 12% ± 8.9%, significantly less than that of random areas (33% ± 3.6%, P = 0.026, Mann-Whitney test).

CONCLUSIONS. Autofluorescent flecks and FIAF deposits with AF levels elevated above the initial macular background were less likely in the short term (2 years) to transform to GA and FDAF (AF levels below the final background) than random areas, suggesting additional mechanisms beyond direct lipofuscin toxicity. FIAF/FDAF levels were observed to fluctuate, with focal remodeling of FIAF and FDAF, or rarely, even transition of FDAF to FIAF. FDAF tended to develop, not coincident with, but adjacent to initial FIAF. Because AF identifies these characteristic biological markers so specifically, autofluorescence metrics merit consideration in the study of STGD.