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A more recent version of this article appeared on September 1, 2009
 (Investigative Ophthalmology and Visual Science. )
© 2009 by The Association for Research in Vision and Ophthalmology, Inc.
doi:10.1167/iovs.09-3458
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Article

Supernormal ERG Oscillatory Potentials in Transgenic Rabbit with Rhodopsin P347L Mutation and Retinal Degeneration

Takao Sakai 1, Mineo Kondo 2*, Shinji Ueno 1, Toshiyuki Koyasu 1, keiichi Komeima 1, and Hiroko Terasaki 1

1 Department of Ophthalmology, Nagoya University Graduate School of Medicine, Nagoya, Japan
2 Department of Ophthalmology, Nagoya University Graduate School of Medicine, 65 Tsuruma-cho, Showa-ku, Nagoya, 466-8550, Japan

* To whom correspondence should be addressed. E-mail: kondomi{at}med.nagoya-u.ac.jp.


  Abstract

Purpose. To determine the properties of the retina of a rhodopsin P347L transgenic (Tg) rabbit model of retinal degeneration by electroretinography (ERG). Methods. Full-field ERGs were recorded from 12- to 48-week-old wild-type (WT) and Tg rabbits. The a-wave was analyzed by the a-wave fitting model of Hood & Birch. The stimulus-response function of the b-wave was analyzed by the Michaelis-Menten equation. Oscillatory potentials (OPs) were extracted by digital filtering after subtracting the a-wave. OPs were also recorded before and after an intravitreal injection of L-2 amino-4-phosphonobutyric acid (APB), cis-2, 3 piperidine dicarboxylic acid (PDA), gamma amino butyric acid (GABA), or tetrodotoxin citrate (TTX). Results. All of the ERG components of Tg rabbits decreased progresively with age with the a-wave more affected than the b-wave, and the OPs were most preserved. Interestingly, the summed OP amplitudes of the Tg rabbits were significantly larger than those of WT rabbits when they were 12-weeks-of-age. The changes in the amplitudes of the OPs after an intravitreal injections of APB, PDA, or GABA in Tg rabbits did not differ significantly from those of WT rabbits. However, injection of TTX resulted in a significantly larger amplitude reduction of the OPs in Tg (65.3%) than in WT (28.6%) rabbits. Conclusion. The significantly larger OPs in Tg rabbits resulted from alterations in the inner retinal neurons. The greater effect of TTX on the OPs amplitudes in Tg rabbits suggests that the supernormal OPs in Tg rabbits may be related to secondary changes in the spiking neurons of the inner retina after the photoreceptor degeneration.

Key Words: retinal degeneration, electroretinography, rhodopsin, oscillatory potentials, Rabbit, Animal model






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