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P<P, published online ahead of print October 15, 2009
(Investigative Ophthalmology and Visual Science. )
© 2009 by The Association for Research in Vision and Ophthalmology, Inc.
doi:10.1167/iovs.09-4134
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Sustained mechanical release of dexamethasone sodium phosphate from a foldable capsular vitreous body

Yaqin Liu,1 Qichen Ke,2 Jiajia Chen,3 Zhichong Wang,4 Zhiyong Xie,5 Zhaoxin Jiang,6 Jian Ge,7 and Qianying Gao8

1State Key Laboratory of Ophthalmology, Zhongshan Ophthalmic Center, Sun Yat-sen University, Guangzhou, China 2State Key Laboratory of Ophthalmology, Zhongshan Ophthalmic Center, Sun Yat-sen University, Guangzhou, China 3State Key Laboratory of Ophthalmology, Zhongshan Ophthalmic Center, Sun Yat-sen University, Guangzhou, China 4State Key Laboratory of Ophthalmology, Zhongshan Ophthalmic Center, Sun Yat-sen University, Guangzhou, China 5Laboratory of Pharmaceutical Analysis & Quality Assessment, School of Pharmaceutical Sciences, Sun Yat-sen University, Guangzhou, China 6State Key Laboratory of Ophthalmology, Zhongshan Ophthalmic Center, Sun Yat-sen University, Guangzhou, China 7State Key Laboratory of Ophthalmology, Zhongshan Ophthalmic Center, Sun Yat-sen University, Guangzhou, China 8State Key Laboratory of Ophthalmology, Zhongshan Ophthalmic Center, Sun Yat-sen University, Guangzhou, China

Correspondence: Qianying Gao, Email: gaoqy{at}hotmail.com

Abstract

PURPOSE: Since 300 nm mili apertures existed in the capsule of the foldable capsular vitreous body (FCVB), we tested if the FCVB could mechanically release dexamethasone sodium phosphate (DexP) from its capsule.

METHODS: In the in vitro study, DexP at concentrations of 0.25, 0.5, 1, 2, and 4 mg/ml in a balanced salt solution were injected into the capsules, which were immersed in cups of modified Franz diffusion cells. Two hundred (200) µL liquid was aspirated at time intervals of 10, 20, 40, 60, 120, 180, 240, 300, and 360 min. In the in vivo study, the capsule was folded and implanted into the vitreous cavity of 5 rabbits. About 0.6 ml of DexP (2 mg/ml) was then injected into the capsule. An intravitreal injection with DexP was performed on another 5 rabbits as the control group. Aqueous humor was aspirated on days 1, 3, 7, 14, 28, and 42 after implantation. The DexP contents in the cups and aqueous humor were detected by HPLC-MS/MS method.

RESULTS: FCVB released DexP in a time-dependent and dose-dependent manner in vitro with five dosages from 10 to 360 min. Especially in the 0.25 mg/ml of DexP group, the content (y) had good linear relationships with time (x), y=0.7635x+10.205. The DexP contents in the aqueous humor were detected until day 28 and were undetectable on day 42. However, the DexP contents were detected only before day 3 in the controls.

CONCLUSIONS: FCVB can sustainably, mechanically release DexP via capsule apertures in a time-dependent and dose-dependent manner in addition to serving as a vitreous substitute.

Key Words: drug delivery • vitreoretinopathy • retinal detachment






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