| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH | TABLE OF CONTENTS |
|
|
|
|||||||
|
|||||||||
1From the Departments of Biomedical Engineering and 2Neurobiology and Physiology and the 3Institute for Neuroscience, Northwestern University, Evanston, Illinois.
 |
Abstract |
|---|
 Top Abstract MethodsResultsDiscussionReferences |
|---|
METHODS. Spatial profiles of oxygen tension were obtained in the cat retina, in vivo, with the use of oxygen microelectrodes during control conditions and acute (5.19 ± 0.83 hour) episodes of hyperglycemia (blood glucose, >350 mg/dL). The outer retinal portions of the profiles were fitted to a model of oxygen diffusion to quantify photoreceptor oxygen consumption.
RESULTS. Photoreceptor oxygen consumption did not significantly change during hyperglycemia compared with control conditions. Choroidal PO2 decreased during hyperglycemia by an average of 5.8 ± 7.4 mm Hg. This led to an increase in the fraction of O2 used by the photoreceptors that was derived from the inner retina. Choroidal PO2 did not recover when blood glucose levels were returned to normal. Average inner retinal PO2 was not affected by the episodes of hyperglycemia.
CONCLUSIONS. The Crabtree effect does not occur to any significant degree in the outer retina, because hyperglycemia did not affect photoreceptor oxygen consumption. Choroidal PO2 decreased during hyperglycemia, and the oxygen deficit was made up by the retinal circulation.
Cohen and Noell7 did not observe a Crabtree effect in rabbit photoreceptors. They found the effect in the inner retina of adult rabbits and in young rabbits before the photoreceptors were fully developed, but this may not be relevant to the more highly vascularized feline or human inner retina. In support of the independence of outer retinal oxidative and glycolytic metabolism, an enzyme-localization study showed that lactate dehydrogenase (LDH), the enzyme that converts pyruvate to lactate, is present primarily in areas without mitochondria in the photoreceptor in the monkey retina.8
In contrast, Van den Enden et al.9 showed a 31% increase in lactate production in the isolated rat retina when the glucose concentration in the bath solution was increased from 5 to 20 mM. An increase in lactate production of 25% was confirmed by Winkler et al.10 when the experiments were repeated. However, they also observed a greater tissue ATP content in the retina bathed in the high-glucose medium.10 Because the ATP content in 20 mM glucose was the same as that observed in the intact rat retina, they concluded that 5 mM did not provide the isolated retina with enough glucose to support normal metabolism. They suggested that more glucose is needed in vitro, because there is an increased diffusional resistance, probably resulting from the presence of unstirred layers adjacent to the tissue and a nonperfused retinal circulation. Thus, the increased glycolysis was not considered evidence of a Crabtree effect.
In long-term hyperglycemia (diabetes), indirect evidence has been obtained in support of a retinal Crabtree effect. Rimmer and Linsenmeier11 found that the RPE c-wave was more resistant to hypoxia in diabetic rats than in healthy control animals. One interpretation of this finding was that the diabetic rats have an increased reliance on glycolysis. Linsenmeier et al.12 have also observed a decrease in outer retinal O2 consumption (QO2) in long-term diabetes in cats. However, this decrease in QO2 most likely resulted from the decreased choroidal PO2 that was observed in the study, rather than the Crabtree effect. QO2 is positively correlated with choroidal PO2.13 14 It is also possible, but less likely, that an adaptive mechanism in the choroid, as a consequence of a decreased O2 demand, lowered choroidal PO2.
In the current set of experiments, we directly examined outer retinal oxidative metabolism during normoglycemia and acute hyperglycemia to determine whether the Crabtree effect occurs in the intact cat retina. In addition, measurements during hyperglycemia may give insight into processes occurring during diabetes. The outer (distal) portion of the intraretinal O2 tension (PO2) profile was fitted to a diffusion model to quantify photoreceptor O2 consumption. Profile parameters were also used to examine other aspects of oxidative metabolism in the outer retina. The inner retinal portion of PO2 profiles allowed the examination of inner retinal oxygenation and O2 autoregulation during hyperglycemia.
|
Methods |
|---|
|
TopAbstractMethodsResultsDiscussionReferences |
|---|
Two saphenous veins and one femoral artery were cannulated for drug delivery and blood pressure monitoring, respectively. A tracheotomy was also performed to allow artificial respiration after induction of paralysis when all surgical procedures were completed.
The cat was placed on its back in a Faraday cage. The head was secured in a head holder with the eyes facing upward. An incision, approximately 3 in. long, was made from the lateral canthus toward the ear. The underlying fascia and muscle were resected to cleanly expose the cartilage and bone of the orbit. The nictitating membrane was clamped and removed. Enough bone and cartilage were dissected to accommodate the electrode manipulator. The fascia and muscle on the orbit itself were resected, and the eye was hung on an eye ring by the conjunctiva for stabilization. The animal was paralyzed by an intravenous infusion of pancuronium bromide (2 mg). Both pancuronium bromide–induced paralysis and anesthesia with urethane were continued for the remainder of the experiment, with maintenance doses of 0.2 mg/kg per hour and approximately 25 mg/kg per hour, respectively. The animal was artificially ventilated at a rate and volume suitable for maintaining arterial blood gas levels within the normal limits (i.e., PaO2 >90 mm Hg, PaCO2 
30 mm Hg, 7.35 < pHa < 7.45). Blood gas levels, glucose, and lactate were measured with a blood gas analyzer (model 860; Bayer/Ciba-Corning, Norwood, MA).
A 15-gauge needle was then placed through the sclera into the vitreous humor. A calibrated oxygen-voltage microelectrode was placed through the needle lumen and attached to a hydraulic microdrive (David Kopf, Tujunga, CA) to allow micrometer control of the electrode position. A Silastic (T-2; Dow Corning, Midland, MI) boot system aided in preventing vitreous leakage and loss of intraocular pressure. In addition, two 20-gauge needles were placed through the sclera for the insertion of polyethylene (Intramedic PE 10; BD Biosciences, Parsippany, NJ) insulated vitreal Ag/AgCl wires. One of these was used as the reference for the oxygen electrode; the other was used for recording the vitreal ERG. Another Ag/AgCl electrode was sutured into the fascia in the head and served as a common reference for both the vitreal electrode and the voltage barrel of the microelectrode.
Oxygen-Voltage Microelectrode
Recessed, polarographic double-barreled oxygen electrodes15 were used. The oxygen-sensing barrel consisted of a gold cathode, polarized at a constant voltage (-0.7V) in comparison with an Ag/AgCl reference. The electrode was calibrated immediately before use in a saline-filled tonometer bubbled with gases at a known PO2. Three calibration points were taken at 0%, 4%, and 8% O2 (PO2 = 0, 28, and 56 mm Hg, respectively). The electrodes produced a current on the order of a few picoamperes, which was linear in its relation to the PO2 at the electrode’s tip. The current was measured by a picoammeter (614 Electrometer; Keithley Instruments, Cleveland, OH) which converted the current to a voltage. The second barrel, filled with 0.9% NaCl, acted as a voltage electrode to record local intraretinal ERGs. Overall electrode tip diameters ranged from 5.0 to 6.5 µm with cathode diameters ranging from 1 to 2 µm. The sensitivity of the electrodes was on the order of 10-13 to 10-14 A/mm Hg.
Stimuli
ERGs were recorded in response to a 4-second flash of diffuse white light. Light stimuli were produced by a tungsten iodide H1 automobile headlamp bulb, which had a maximum illumination of 9.4 (equivalent log quanta [555 nm] per degree squared per second). The light source was contained in an optical bench located outside the Faraday cage and was brought to the cat through a fiber-optic bundle. A neutral-density wedge filter was used to attenuate the light source. A timer (A-65 Timer/Stimulator; Winston Electronics Co., San Francisco, CA) controlled the light shutter (ST-2 shutter driver; Winston Electronics Co., San Francisco, CA; Uniblitz shutter; Vincent Associates, Rochester, NY).
Data Collection
The oxygen current was recorded from the oxygen barrel, whereas local ERGs, referenced to a scalp Ag/AgCl electrode, were collected from the voltage barrel. The retina was penetrated by advancing the microelectrode in 3-µm steps. Local ERGs were recorded every 30 µm to assess the position of the electrode in the retina. On reaching the choroidal circulation, as indicated by high oxygen levels and the transepithelial potential across the RPE, the electrode was withdrawn at a steady 2 µm/sec while continuously recording the oxygen current, generating a profile of PO2 as a function of position. For analysis of the profiles, the position of the RPE was taken to be the point at which PO2 began to decrease, and the position of the vitreoretinal border was taken to be the point at which the ERG recorded with the microelectrode and the ERG recorded with the vitreal electrode were the same. Several normoglycemic profiles (blood glucose, 80–120 mg/dL) were recorded under dark-adapted conditions. The cat was then made hyperglycemic (blood glucose >350 mg/dL) through continuous intravenous infusion of 40% glucose. Whenever possible, blood glucose was returned to the normal range with continuous intravenous infusion of insulin, and recovery profiles were collected. Recovery from hyperglycemia was achieved in five of the nine cats.
Modeling of Outer Retinal Oxygen Tension
A three-layer diffusion model, developed and fully described by Haugh et al.16 and Braun et al.17 , was fitted to the outer half of intraretinal PO2 profiles, corresponding to the avascular portion of the retina. Briefly, the outer retina was divided into three anatomic layers: the RPE and photoreceptor outer segments (OS), the photoreceptor inner segments (IS), and the outer nuclear layer and outer plexiform layer (ONL-OPL). Because mitochondria are localized to the photoreceptor IS, oxygen consumption is nonzero only in the IS layer (Fig. 1) . The solution to the O2 diffusion model is an equation for PO2 as a function of distance (x) from the choroid that is linear in nonconsuming regions (OS, ONL) and quadratic in the consuming region (IS). PC is the fitted PO2 at the choroid–RPE boundary. PL is the fitted PO2 at the inner–outer retinal border (x = L), which was taken to be 50% of retinal depth. The main parameter derived from fitting is QOR-O2, the oxygen consumption of the outer half of the retina. QOR-O2 was corrected to compensate for the differences in profile length, due to possible tissue distortions that may occur during electrode withdrawal, by multiplying the fitted QOR-O2 by (L/LOR),2 where L is the outer retinal thickness, in micrometers, of the individual profiles, and LOR is the average thickness of many outer retinal profiles. LOR is the actual thickness of the outer retina, taken to be 100 µm in the current study, based on published data.13 14 A more detailed discussion of this correction factor can be found in Yancey and Linsenmeier.13 A satisfactory model fit to the data could not be obtained in one cat.
|
Statistics
Individual profile values were averaged within cats to obtain an average parameter value for each cat. Two-tailed paired t-tests were performed to determine whether there was a significant difference between control and altered conditions. This method ensured that data from each cat were weighted equally. P < 0.05 was defined as statistically significant. The average results from each cat were then averaged to obtain the overall average reported in this study. All data are reported as the mean ± SD.
|
Results |
|---|
|
TopAbstractMethodsResultsDiscussionReferences |
|---|
|
|
During hyperglycemia, the measured choroidal PO2 (PC) decreased by an average of 5.8 ± 7.4 mm Hg, but by as much as 21.2 mm Hg in one cat. Actual choroidal PO2 was 41.2 ± 16.2 mm Hg during normoglycemia and 35.4 ± 11.8 during hyperglycemia (n = 9, P = 0.049). Individual profiles demonstrating the hyperglycemia-induced decrease in choroidal PO2 are shown in Figure 2 for two cats. The normalized change in choroidal PO2 for each cat and the time course in one cat are shown in Figure 4 . Because of the lowered choroidal PO2, a larger fraction of oxygen was delivered to the photoreceptors by the retinal circulation during hyperglycemia compared with normoglycemia. Figure 5 shows that the amount of oxygen delivered to the photoreceptors by the retinal circulation increased by 57% during hyperglycemia from 7.4% ± 5.9% during normoglycemia to 11.6% ± 6.3% during hyperglycemia (P = 0.03, n = 8).
|
|
Effect of Acute Hyperglycemia on Inner Retinal PO2
The average inner retinal PO2 did not change significantly during hyperglycemia (12.1 ± 4.4 mm Hg, n = 9) compared with normoglycemia (12.5 ± 4.5 mm Hg, n = 9). Preretinal PO2 (0–100 µm away from the retinal surface) was also examined, with similar findings. In the 100 µm of vitreous adjacent to the retina, average PO2 was 15.6 ± 6.5 mm Hg during normoglycemia and 13.6 ± 5.8 mm Hg during hyperglycemia.
Data were recorded from five cats during the recovery period and also showed no significant change in average inner retinal PO2 or preretinal PO2 when blood glucose levels were returned to normal.
|
Discussion |
|---|
|
TopAbstractMethodsResultsDiscussionReferences |
|---|
There is some evidence that long-term hyperglycemia changes retinal metabolism, however. Illing and Gray19 and Sutherland et al.20 observed a decrease in oxygen consumption in the diabetic rabbit retina compared with normal animals. Because the rabbit retina is mostly avascular, total retinal consumption is representative of outer retinal (photoreceptor) oxygen consumption. Therefore, the results of the present study may indicate that decreased photoreceptor oxygen consumption in diabetes is a secondary change, rather than one resulting directly from hyperglycemia.
In the current work, QOR-O2 was lower than in some previous studies. The average QOR-O2 during normoglycemia was 3.03 ± 1.41 mL O2/100 g per minute compared with a range of 3.9 to 5.1 mL O2/100 g per minute in previous studies.14 15 16 17 This is most likely a direct result of PC’s being lower than usual in this study (40.9 ± 16.7 mm Hg vs. >60 mm Hg). In addition, the large variation of QOR-O2 in this study can be explained by the wide variation in PC. In agreement with previous work,12 14 QOR-O2 had a strong linear relationship with PC (Fig. 6) .
|
The decrease in choroidal PO2 during hyperglycemia was not a general deterioration with time, but was related to blood glucose levels, as is shown in Figure 4B . Because of the direct relationship between choroidal PO2 and QOR-O2,14 QOR-O2 would also be expected to decrease. The decreased PC during hyperglycemia, however, did not cause a further reduction in QOR-O2. This may be partially because our method is not sensitive to very small changes in QOR-O2, but also that the O2 deficit from the decreased choroidal oxygen flux seemed to be made up by the retinal circulation. This was indicated by the 57% increase in photoreceptor oxygen derived from the retinal circulation (11.6% vs. 7.4%). Because the hyperglycemia-induced decrease in PC did not correlate with an increase in average inner retinal PO2, it seems that the increased reliance of the photoreceptors on the inner retina was not a regulatory compensation by the retinal circulation that was related to the decreased choroidal PO2. Rather, the increased reliance of the photoreceptor on the retinal circulation may be passively related to the acidosis that occurs during acute hyperglycemia.18 One consequence of acidosis may be the 15% increase in retinal blood flow that occurs during hyperglycemia in cats.24 Although our data did not reveal an increase in average inner retinal PO2 during hyperglycemia, which might have been expected with an increase in blood flow, we may have missed increases because inner retinal PO2 is variable and we could not record from identical locations before and during hyperglycemia.
Tiedeman et al.25 observed a 12% decrease in retinal vein O2-saturation during hyperglycemia in diabetic persons with no retinopathy. They interpreted this as an increase in inner retinal oxygen consumption. We cannot rule out this explanation, but it is possible that in these subjects, choroidal PO2 decreased during hyperglycemia, as in this experimental series. If this was the case, an increased flux of oxygen from the retinal circulation to the photoreceptor could result in an increase in the oxygen delivered by the retinal circulation. We suggest that their results do not necessarily indicate an increase in total retinal oxygen consumption, but possibly a redistribution of oxygen, and potentially other nutrients, from the retinal circulation to the outer retina.
The 11.4% decrease in choroidal oxygen tension is probably reflective of a decrease in choroidal blood flow. Consistent with this conclusion, decreased choroidal lobular perfusion has been observed in diabetic patients,21 and one animal in a study in cats with long-term diabetes had a particularly low choroidal PO2 (PC = 15.8 mm Hg).12 It should be mentioned, however, that Ernest and Goldstick26 reported a decreased choroidal vascular resistance during an abrupt increase in blood glucose to 1500 mg/dL for 30 minutes in the cat. Their observations may differ from ours, because the differences in time course and severity of hyperglycemia make the two studies incomparable. The mechanism by which hyperglycemia would change choroidal blood flow in either direction is not currently known.
Effect of Hyperglycemia on the Inner Retina
The effect of hyperglycemia on the inner retina was surprisingly small. Because average inner retinal PO2 did not change significantly, it is reasonable to suggest that hyperglycemia does not hinder autoregulation of inner retinal oxygen during air breathing. However during hyperoxia the situation may be different. Ernest et al.27 observed an increase in preretinal PO2 transients in response to breathing 100% O2 in both normal and diabetic dogs during hyperglycemia compared with normoglycemia. Similarly, Grunwald et al.28 found a smaller regulatory decrease in retinal blood flow in diabetic patients compared with age-matched normal subjects during hyperoxia.
Although short-term hyperglycemia did not produce inner retinal hypoxia, experimental diabetes is known to be accompanied by tissue hypoxia12 29 and altered oxygen flux from retinal arteries.30 The present work does not provide evidence on whether prolonged hyperglycemia per se or other pathologic effects secondary to prolonged hyperglycemia cause the hypoxia in diabetes. We have suggested previously that the activation of leukocytes and consequent plugging of retinal capillaries are among the important changes initiated by hyperglycemia that lead to tissue hypoxia.12
In summary, photoreceptor oxidative metabolism and retinal oxygenation did not drastically change during acute episodes of hyperglycemia in the intact cat retina. These results, along with our study demonstrating retinal pH changes during hyperglycemia,18 concur with other studies that have failed to demonstrate a Crabtree effect in the outer retina.7 Further, we conclude that short-term hyperglycemia does not lead directly to tissue hypoxia in the inner retina.
|
Acknowledgements |
|---|
|
Footnotes |
|---|
Submitted for publication May 1, 2002; revised July 8, 2002; accepted July 16, 2002.
Commercial relationships policy: N.
The publication costs of this article were defrayed in part by page charge payment. This article must therefore be marked "advertisement" in accordance with 18 U.S.C. 
1734 solely to indicate this fact.
Corresponding author: Robert A. Linsenmeier, Biomedical Engineering Department, Northwestern University, 2145 Sheridan Road, Evanston, IL 60208-3107; r-linsenmeier{at}northwestern.edu.
|
References |
|---|
|
TopAbstractMethodsResultsDiscussionReferences |
|---|
This article has been cited by other articles:
|
|
 |
|
  E. D. Gilmore, C. Hudson, R. K. Nrusimhadevara, R. Ridout, P. T. Harvey, M. Mandelcorn, W.-C. Lam, and R. G. Devenyi Retinal Arteriolar Hemodynamic Response to a Combined Isocapnic Hyperoxia and Glucose Provocation in Early Sight-Threatening Diabetic Retinopathy Invest. Ophthalmol. Vis. Sci., February 1, 2008; 49(2): 699 - 705. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
E. Budzynski, J. H. Smith, P. Bryar, G. Birol, and R. A. Linsenmeier Effects of Photocoagulation on Intraretinal PO2 in Cat Invest. Ophthalmol. Vis. Sci., January 1, 2008; 49(1): 380 - 389. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 G. Birol, S. Wang, E. Budzynski, N. D. Wangsa-Wirawan, and R. A. Linsenmeier Oxygen distribution and consumption in the macaque retina Am J Physiol Heart Circ Physiol, September 1, 2007; 293(3): H1696 - H1704. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
S. Wang and R. A. Linsenmeier Hyperoxia Improves Oxygen Consumption in the Detached Feline Retina Invest. Ophthalmol. Vis. Sci., March 1, 2007; 48(3): 1335 - 1341. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 L. Padnick-Silver and R. A. Linsenmeier Effect of Hypoxemia and Hyperglycemia on pH in the Intact Cat Retina Arch Ophthalmol, December 1, 2005; 123(12): 1684 - 1690. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
G. Birol, E. Budzynski, N. D. Wangsa-Wirawan, and R. A. Linsenmeier Hyperoxia Promotes Electroretinogram Recovery after Retinal Artery Occlusion in Cats Invest. Ophthalmol. Vis. Sci., October 1, 2004; 45(10): 3690 - 3696. [Abstract] [Full Text] [PDF]
|
|
| ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH | TABLE OF CONTENTS |
) the average across cats are shown. Error bars, SD.
