Appendix

Xue Chen, Zhuming Ai, Mary Rasmussen, Peter Bajcsy, Loretta Auvil, Michael Welge, Lu Leach, Sumalee Vangveeravong, Andrew J. Maniotis and Robert Folberg

Files in this Data Supplement:

• Video Clip 1 - 6.25 MB

  The 3-D reconstruction from Figure 3G is rotated in real time by the investigator. The image of the hand is the icon of the wand used to manipulate the image. As the entire reconstruction is rotated from side to side, the "space" encircled by laminin and marked with an asterisk in Figure 3G assumes a cylindrical shape. In reality, there is no space because S100-positive tumor cells occupy this compartment. Not staining for the tumor cells makes the 3-D distribution of laminin visible.

• Video Clip 2 - 11.0 MB

  The 3-D reconstruction from Figure 3G is rotated in real time by the investigator. In this reconstruction, the investigator, wearing position-sensitive goggles (Figure 1), has leaned forward toward the ImmersaDesk, rotating the reconstruction back. The image is then rotated from side-to-side with the wand as in video clip 1. The "space" marked with the plus sign (+) in Figure 3G is now the center of attention. Note that what appears to be a clear space from front to back in Figure 3G is actually subdivided by branching laminin-positive septa in this video clip.