Movies

Files in this Data Supplement:

• Movie 1 - 8.52 MB (.mov)
  Time-sequential imaging of vitreoretinal angiogenesis in one retinal explant. Time-lapse confocal images of vascular sprouting in the retinal explant of Tie2-GFP transgenic mouse treated with 25 ng/ml VEGF. The vascular sprouts which have already developed grow, and several new sprouts are emerging. Images were captured at 101 timepoints, beginning at 84 hours after the onset of organ culture. The movie frame rate is 8 frames per second and each frame was taken at 15-minute intervals.

• Movie 2 - 9.53 MB (.mov)
  Time-sequential imaging of vitreoretinal angiogenesis in another retinal explant. Time-lapse confocal images of vascular sprouting in the retinal explant of Tie2-GFP transgenic mouse treated with 25 ng/ml VEGF. The retina other than that in Movie 1 was shown. Images were captured at 109 timepoints, beginning at 96 hours after the onset of organ culture. The movie frame rate is 8 frames per second and each frame was taken at 15-minute intervals.

• Movie 3 - 1.01 MB (.mov)
  The first step during sprouting is a single process extension of one cell. Time-lapse confocal images of vascular sprouting in the retinal explant of Tie2-GFP transgenic mouse treated with 25 ng/ml VEGF. The leading edge of an endothelial cell elongates from mature vessels, followed by the migration of the cell body. Images were collected at 52 timepoints, beginning at 84 hours after the onset of organ culture. The movie frame rate is 8 frames per second and each frame was taken at 15-minute intervals.

• Movie 4 - 505 KB (.mov)
  The leading edges of sprouting endothelial cells extend and retract. Time-lapse confocal images of vascular sprouting in the retinal explant of Tie2-GFP transgenic mouse treated with 25 ng/ml VEGF. The leading edges of sprouting endothelial cells extend and retract one after another and cell body migration then follows. Images were captured at 101 timepoints, beginning at 84 hours after the onset of organ culture. The movie frame rate is 8 frames per second and each frame was taken at 15-minute intervals.

• Movie 5 - 1.27 MB (.mov)
  Branching and fusion of sprouting endothelial cells. Time-lapse confocal images of vascular sprouting in the retinal explant of Tie2-GFP transgenic mouse treated with 25 ng/ml VEGF. From one vascular sprout, another emerges, which results in vascular branching. Two newly formed vessels, but not parent vessels, then fuse to each other. Images were captured at 70 timepoints, beginning at 84 hours after the onset of organ culture. The movie frame rate is 8 frames per second and each frame was taken at 15-minute intervals.