Supplementary Material

Itay Chowers, Robert Wong, Tzvete Dentchev, Ronald H. Farkas, Jared Iacovelli, Tushara L. Gunatilaka, Nancy E. Medeiros, J. Brett Presley, Peter A. Campochiaro, Christine A. Curcio, Joshua L. Dunaief, and Donald J. Zack

Files in this Data Supplement:

• Supplementary Table S1 - 38.6 KB (PDF)

• Supplementary Figure S1 - 110 KB (PDF)
  Post-mortem fundus appearance in eyes fellow to those used for transferrin detection. All panels except B are stereo pairs. B shows one macula under two lighting conditions. (A) Macula with mild post-mortem retinal edema and an apparent defect in the nerve fiber layer (arrow) (oblique trans-scleral illumination, 2003121L, 90-year-old woman). (B) Macula with perifoveal drusen (arrowhead) and geographic atrophy (arrow) (epi-illumination and oblique transillumination, respectively, 2003128L, 87-year-old woman). (C) Macula with RPE atrophy (arrow), pigment clumping, and moderate post-mortem retinal edema (oblique trans-scleral illumination, 2003136L, 74-year-old man). (D) Macula with geographic atrophy of RPE (arrow), overlying deeply pigmented choroid with serpentine empty vessels, and hyperpigmented RPE peripheral to geographic atrophy and in two spots in fovea (arrowhead) (epi-illumination and oblique trans-scleral illumination, 2004016R, 85-year-old woman). (E) Macula with geographic atrophy of RPE, overlying deeply pigmented choroid, with serpentine empty vessels, and translucent fibrovascular scar (asterisk) (epi-illumination and oblique trans-scleral illumination, 2004023L, 98-year-old man). Bar, 1 mm.

• Supplementary Figure S2 - 921 KB (PDF)
  Lesion ultrastructure in eyes fellow to those used for transferrin detection I. (A) Normal aged Bruch's membrane (2003121L, 90-year-old woman). R, RPE; double arrowhead, RPE basal lamina; ChC-E, choriocapillary endothelial cell; arrow, banded material with 50 nm periodicity; box, lipoprotein-like particles with external projections resembling gears; p, plasma; bar, 1 mm. (B) Eye with late non-neovascular AMD (2003128L, 87-year-old woman) has 2 coarse bands and 3 fine bands typical of type VI collagen; bar, 200 nm. (C) Same eye as B (2003128L, 87-year-old woman) has basal laminar (lam) and linear (lin) deposit with abundant membranous debris (white arrow, without contents; black arrow, with contents); box, type VI collagen shown in panel B; double arrowhead, RPE basal lamina; Br, Bruch's membrane, bar, 1 mm.

• Supplementary Figure S3 - 717 KB (PDF)
  Lesion ultrastructure in eyes fellow to those used for transferrin detection II. (A) Eye with late neovascular AMD (2004016R, 85-year-old woman) containing basal laminar deposit. Arrows denote banded material that differs from type VI collagen. (B) Eye with late neovascular AMD (2004023L, 98-year-old man) has extravasated macrophages (M) within Bruch's membrane. (C, D) Same eye as B (2004023L, 98-year-old man) has membranous debris within basal laminar deposit, with (black arrow) and without (white arrow) contents; asterisks denote pools of presumed neutral lipid. Bar, 1 mm in A applies to all.