Supplementary Figure S1
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(JPG)
Colony-forming efficiency of HLEC seeded at various densities in normoxic and hypoxic culture. Hypoxic colonies were larger than normoxic colony in all seeding densities (A, rhodamine; B, stained dishes). Colony-forming efficiency in 300, 500, and 1000 cells/dish seeding densities were 1.1 ± 1.0 % under normoxia and 2.8 ± 1.6 % under hypoxia, 1.2 ± 0.5 % in normoxia and 2.6 ± 1.6 % in hypoxia, and 1.3 ± 0.6 % in normoxia and 2.6 ± 1.6 % in hypoxia, respectively. Difference in CFE reached statistical significance at densities of 300 and 1000 cells/dish (B: P < 0.05, paired t-test, n = 5).
Supplementary Figure S2
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(JPG)
Cell densities of hypoxic and normoxic colonies. Cultures were fixed when colonies reached at same approximate size (A, B), and nuclei were stained with DAPI (C, D). Cell density of hypoxic colony (625.9 ± 136.4 cells per mm2, mean ± S.D., n = 20) was significantly higher than normoxic culture (478.1 ± 149.0 cells per mm2) (P < 0.01, paired t-test). Scale bar, 200 mm.
