IOVS
HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
QUICK SEARCH:   [advanced]


     

(Investigative Ophthalmology and Visual Science. 2008;49:1362-1366.)
© 2008 by The Association for Research in Vision and Ophthalmology, Inc.
doi:10.1167/iovs.07-0150
This Article
Right arrow Abstract Freely available
Right arrow Full Text (PDF)
Right arrow Submit a response
Right arrow Alert me when this article is cited
Right arrow Alert me when eLetters are posted
Right arrow Alert me if a correction is posted
Right arrow Citation Map
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in Web of Science
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrow reprints & permissions
Citing Articles
Right arrow Citing Articles via Web of Science (3)
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Rochtchina, E.
Right arrow Articles by Mitchell, P.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Rochtchina, E.
Right arrow Articles by Mitchell, P.

Ethnic Variability in Retinal Vessel Caliber: A Potential Source of Measurement Error from Ocular Pigmentation?—The Sydney Childhood Eye Study

Elena Rochtchina,1 Jie Jin Wang,1,2 Bronwen Taylor,1 Tien Y. Wong,2,3 and Paul Mitchell1

1From the Centre for Vision Research, Department of Ophthalmology, Westmead Millennium Institute, University of Sydney, Sydney, Australia; the 2Centre for Eye Research Australia, University of Melbourne, Melbourne, Australia; and the 3Singapore Eye Research Institute, Yong Loo Lin School of Medicine, National University of Singapore, Singapore.


   Abstract
Top
 Abstract
Methods
 Results
 Discussion
 References
 
PURPOSE. To describe the effects of ethnicity and iris color on measurement of retinal vascular caliber.

METHODS. The Sydney Childhood Eye Study (SCES) examined 1740 6-year-old children from 34 randomly selected Sydney schools during 2003 and 2004. Retinal arteriolar and venular calibers (central retinal arteriolar [CRAE] and central retinal venular [CRVE] equivalents) were measured from retinal photographs by a standardized computer-assisted method. Associations of retinal vascular caliber with ethnicity and iris color were analyzed.

RESULTS. Of the total participants, 1612 (92.6%) had gradable retinal photographs. CRAE and CRVE were normally distributed, with means (SD) of 163.2 (14.0) and 227.3 (18.3) µm, respectively. Both arteriolar and venular calibers were substantially wider in the East Asian than in the Caucasian children (CRAE, 171.5 µm vs. 160.5 µm; and CRVE, 240.5 µm vs. 222.4 µm; P < 0.0001 for both comparisons). Among the Caucasian children, darker iris color was associated with wider arteriolar and venular caliber: CRAE increased from 157.5 µm in blue eyes to 169.2 µm in dark brown eyes (P for trend < 0.0001), and CRVE increased from 218.4 µm in blue eyes to 230.0 µm in dark brown eyes (P for trend < 0.0001). Among the children with dark brown eyes, there was no significant difference in CRAE between the Caucasian and the East Asian children, although mean CRVE was still approximately 10 µm wider in the East Asian than in the Caucasian children.

CONCLUSIONS. Retinal vessel caliber was wider in the children of East Asian ethnicity. This difference was explained principally by darker iris color. Retinal pigmentation, as approximated by iris color, could be an important source of error in the measurement of retinal vessel caliber. If confirmed, this may affect comparisons of retinal vessel caliber between subjects with different ethnic backgrounds.

Retinal vessels provide clinicians and researchers with a unique opportunity to view and characterize the microvascular system noninvasively. Recently developed methods of measuring retinal vascular caliber from retinal photographs1 2 3 have been used in several studies in adult populations,4 5 showing that these measurements are predictive of various cardiovascular diseases.6 7 8 9

There is increasing evidence that retinal vascular caliber may vary between racial and ethnic groups. In the Multi-Ethnic Study of Atherosclerosis (MESA), the blacks and Hispanics had larger retinal arteriolar and venular caliber than the whites and Chinese, despite adjustment for cardiovascular risk factors.5 The Singapore Cohort Study of Risk Factors for Myopia (SCORM) also found significant differences in retinal vascular caliber in Malay, Indian, and Chinese children.10 The underlying reasons for these racial and ethnic differences are unclear and were thought possibly to reflect variations in levels and susceptibility to vascular risk factors (e.g., blood pressure), anthropometric and ocular biometry measures (e.g., axial length), and/or genetic factors.11 12

We now hypothesize that variations in contrast between retinal background color and the vessel edge affect retinal vessel caliber measurements from fundus photographs, and that variations in retinal pigmentation may explain some of the observed racial and ethnic differences. Figure 1 shows fundus photographs from two SCES children. The top photograph is from a blue-eyed Caucasian child and the middle photograph is from a dark brown-eyed East Asian child. The graph shows the pixel density curves over the marked arterioles. The contrast between the vessel edge and retinal background was greater in the blue eye than in the dark brown eye. The slope of the pixel density curve at the vessel edge in the blue eye was also steeper than that in the dark brown eye. As the measurement takes the mid-half value from the curve,13 the gentler slope would give a wider absolute value for this measure.


Figure 1
View larger version (40K):
[in this window]
[in a new window]

  		FIGURE 1. Fundus photographs from (A) a blue-eyed Caucasian child and (B) a dark-brown–eyed East Asian child. (C) Pixel density curves over the marked arterioles.

 
In the present study, we used a semiautomated computer program to assess the variations in retinal vessel caliber, as measured from digital retinal images, by ethnicity and by iris color, a surrogate measure of retinal pigmentation, in a population-based sample of young, school-aged children. Children are the ideal population in which to test this hypothesis, as they are generally free from cardiovascular and ocular diseases.


   Methods
Top
 Abstract
 Methods
Results
 Discussion
 References
 
Study Population
The Sydney Childhood Eye Study is a population-based survey of eye health in school children residing in the metropolitan area of Sydney, Australia. This article describes data from the Sydney Myopia Study component, which examined grade one school children during 2003 and 2004. The study was approved by the Human Research Ethics Committee, University of Sydney and the Department of Education and Training, New South Wales, Australia, and adhered to the tenets of the Declaration of Helsinki. Informed written consent was obtained from at least one parent, as well as verbal assent from each child. Detailed study methods have been described elsewhere.14 15 In brief, 34 primary schools across the Sydney metropolitan region were selected through random cluster sampling, stratified on socioeconomic status. A proportionate mix of government-funded and private/religious schools was included.

Demographic Data
Demographic data were obtained from a comprehensive 193-item questionnaire sent to parents. Questions covered topics including socioeconomic status, medical and ocular history, and birth history (including birth weight and gestational age). Each child’s ethnicity was determined from the ethnicity of both parents. Ethnic groups represented were European Caucasian, East Asian, South Asian (Indian/Pakistani/Sri Lankan), African, Melanesian/Polynesian, Middle Eastern, Indigenous Australian, and South American.

Ocular Examination and Retinal Photography
Iris color was assessed from the undilated pupil of each eye and graded by the observer as blue, hazel-green, tan-brown, or dark brown.

Axial length was measured before cycloplegia with an optical biometer (IOLMaster; Carl Zeiss Meditec, Oberkochen, Germany), using dual-beam partial-coherence interferometry (PCI).16 The average of five measurements was used for analysis.

Cycloplegia was induced by instilling cyclopentolate 1% and tropicamide 1%, 2 drops each, separated by 5 minutes. Phenylephrine 2.5% was also instilled in a small proportion of children, to achieve adequate mydriasis (≥6 mm). Autorefraction (RK-F1 autorefractor/keratometer, Canon, Tokyo, Japan) was performed 25 to 30 minutes after the last drop. Five autorefractions were performed automatically. The median measurement obtained by the instrument was used for analyses.

Mydriatic 60° digital photographs of the optic disc and macula of both eyes were taken with a fundus camera (model 60UVI-D10; Canon). Methods used to measure and summarize retinal vascular caliber from the digital retinal photographs have been described elsewhere.1 17 18 Briefly, a computer imaging program (IVAN; University of Wisconsin, Madison, WI) was used to measure the caliber of all retinal vessels located one half to one disc diameter from the optic disc margin in the digital retinal photograph. Using the Knudtson-Hubbard formula,3 we summarized these measurements as central retinal arteriolar (CRAE) and central retinal venular equivalents (CRVE), representing the average arteriolar and venular calibers in the examined eye, respectively. A single grader (BT), masked to participant characteristics, performed all retinal measurements.18 High correlation between the right and left eyes in retinal vascular caliber measurements has been reported.19 Data from the right eyes were used. High intra- and intergrader reproducibility of the retinal vascular measurements obtained with a computer-based program has been reported.1 2

Anthropometric and Blood Pressure Measurements
Anthropometric measurements including height, weight, and waist circumference, were collected for each child.18 Height without shoes was measured with a freestanding height rod (model 220; Seca, North Rocks, NSW, Australia). Weight was measured with an electronic scale (model TBF-300; Tanita, Tokyo, Japan). Body mass index (BMI) was calculated as weight in kilograms divided by height in meters squared.

Sitting blood pressure and heart rate were measured in the right arm with an oscillometric blood pressure recorder (HEM-907; Omron, Sydney, NSW, Australia).18 After the child had 10 minutes of quiet rest, a single measure of blood pressure was taken using an appropriately sized cuff. Mean arterial blood pressure (MABP) was calculated as one third of the systolic (SBP) plus two thirds of the diastolic blood pressure (DBP).

Statistical Analysis
Analyses were performed for the right eye data (SAS ver. 9.1; SAS Institute, Cary, NC). {chi}2 and t-tests were used to compare selected characteristics between subgroups. Multivariate adjusted means for retinal vascular caliber in subgroups were calculated using analysis of covariance. Mixed models were used for additional adjustment for cluster-sampling effects.


   Results
Top
 Abstract
 Methods
 Results
Discussion
 References
 
Of the 1740 examined children, 1612 (92.6%) had gradable retinal photographs, including 795 (49.3%) girls and 817(50.7%) boys. The mean age of participants was 6.7 years. The ethnic composition of children was predominantly European Caucasian (n = 1023, 63.5%) and East Asian (n = 272, 16.9%). There were also 78 (4.8%) Middle Eastern and 38 (2.4%) South Asian children. The remaining 201 (12.5%) children belonged to five other ethnic categories too low in number for their data to be reported separately.

Table 1 presents selected characteristics of the study population stratified by the two main ethnic groups. The East Asian children were younger and shorter and had lower BMI, lower birthweight, and more myopic refraction than did the European Caucasian children. Almost all (99.3%) of the East Asian children and only 5.5% of the Caucasian children had dark brown iris color. There was no difference in SBP, DBP, and axial length between these two ethnic groups.


View this table:
[in this window]
[in a new window]

  		TABLE 1. Characteristics of the Study Participants

 
The mean retinal arteriolar caliber (CRAE) was normally distributed (mean ± SD of 163.2 ± 14.0 µm; range, 124.5–207.3). The mean retinal venular caliber (CRVE) was also normally distributed (mean ± SD of 227.3 ±18.3 µm; range, 175.3–288.6 µm). Figure 2 shows the distribution of CRAE and CRVE in both the Caucasian and East Asian children.


Figure 2
View larger version (12K):
[in this window]
[in a new window]

  		FIGURE 2. Distribution of (A) retinal arteriolar caliber (CRAE) and (B) retinal venular caliber (CRVE) in European Caucasian and East Asian children in the Sydney Childhood Eye Study (n = 1612).

 
Table 2 shows the mean retinal arteriolar and venular caliber by age, gender, and ethnic group. There was no difference in retinal vascular caliber between the 6- and 7-year-old children. Boys had slightly narrower retinal vascular caliber than did the girls (P < 0.0001 for both calibers), but this difference became nonsignificant after adjustment for other confounding factors, including age, ethnicity, birthweight, height, BMI, MABP, and axial length.


View this table:
[in this window]
[in a new window]

  		TABLE 2. Mean Retinal Arteriolar and Venular Calibers by Age, Gender, and Ethnicity

 
The East Asian, South Asian, and Middle Eastern children all had significantly wider measured retinal arteriolar and venular caliber than did the the European Caucasian children, with the greatest difference (~10 µm CRAE, 20 µm CRVE) observed between the East Asian (mean CRAE, 171.5 µm; mean CRVE, 240.5 µm) and Caucasian children (mean CRAE, 160.5 µm; mean CRVE, 222.4 µm), P < 0.0001 for both calibers, after multivariate adjustment.

Among the European Caucasian children, darker iris color was associated with wider arteriolar and venular caliber (Table 3) . Multivariate adjusted mean CRAE increased from 157.5 µm in blue eyes to 169.2 µm in dark brown eyes (P for trend < 0.0001). Multivariate adjusted mean CRVE increased from 218.4 µm in blue eyes to 230.0 µm in dark brown eyes (P for trend < 0.0001). The findings were similar when children from all ethnic groups were included (Table 3) .


View this table:
[in this window]
[in a new window]

  		TABLE 3. Mean Retinal Arteriolar and Venular Caliber by Iris Color

 
We assessed the ethnic differences in retinal vascular caliber only in the children with dark brown eyes, as this was the only iris color group that included all ethnicities (Table 4) . In this comparison, there was no significant difference in CRAE between European Caucasian and East Asian children (P = 0.24), but the mean CRVE was still approximately 10 µm wider in the East Asian than in the Caucasian children (P = 0.0004). Both caliber measures were not significantly different in the South Asian and Middle Eastern children, when compared with the Caucasian children.


View this table:
[in this window]
[in a new window]

  		TABLE 4. Mean Retinal Arteriolar and Venular Caliber in Children with Dark Brown Iris Color, by Ethnicity

 

   Discussion
Top
 Abstract
 Methods
 Results
 Discussion
References
 
In this study, we found significant differences in retinal vascular caliber, as measured using a semiautomated computer program, in children of different racial and ethnic groups and in eyes of different iris colors. The East Asian children and those European Caucasian children with darker iris color had wider retinal arteriolar and venular caliber. To elucidate the reasons behind the ethnic differences in retinal vessel caliber and to differentiate "ethnicity" from "iris color," we examine this association in the subgroup of Caucasian children with different iris color. The magnitude of the association with iris color among European Caucasian children remained very strong (~10 µm difference in the mean arteriolar and venular caliber between blue eyes and dark brown eyes). Thus, we suggest that the racial–ethnic variation in retinal vessel caliber is principally explained by darker iris color, a proxy for retinal pigmentation.

We further tested our hypothesis using data from the Blue Mountains Eye Study (a population that included 98% European Caucasian adults aged 49+ years) and found similar association between iris color and retinal vascular caliber, although a different computer program, Retinal Analysis,13 was used for the measurements. Both retinal arteriolar and venular calibers were significantly wider (~5 µm difference for both) in persons with dark brown irises than in those with blue irises (P < 0.0001 after controlling for age, gender, BMI, smoking and MABP; Rochtchina E, unpublished data, 2007).

Our study is consistent with and provides a new explanation for the findings in two other studies that examined racial and ethnic differences in retinal vascular caliber. In the MESA study population, the African-Americans and Hispanics, who have darker fundus pigmentation, were reported to have wider mean arteriolar and venular calibers compared with that of the whites and Chinese. Also, the Chinese had wider mean venular caliber than did the whites.5 The SCORM data showed that both retinal arterioles and venules were wider in the Malay or Indian children (who have somewhat darker retinal pigmentation) than in the Chinese children, even after adjustment for age, gender, refraction, and other factors.10 Darker iris color correlates highly with non-Caucasian ethnicity. In our sample, among children with dark brown eyes, only 11% were of European Caucasian ethnicity. We therefore propose that the observed ethnic differences in retinal caliber are likely to be at least partly explained by the different retinal pigment background indicated by various iris colors. Our findings from subgroup analyses of children with dark brown iris color support this hypothesis. Among children with dark brown eyes, we found no difference in the mean arteriolar caliber between European Caucasian and East Asian children. The difference in the mean venular caliber remained significant, with a reduced magnitude (Table 4) .

Our study suggests that a higher level of retinal pigmentation (represented by darker iris color) may reduce the contrast between the background and retinal vessels, making the vessel edge harder to detect with the semiautomatic computer program. This seems likely to result in measurements that exceed the true boundary of the vessel. We are currently planning a fluorescein angiographic comparison study for investigation of this hypothesis.

We should consider alternative hypotheses. It is possible, for example, that the observed relationship between retinal vessel caliber with iris color is in part attributable to genetic differences.20 However, we have no means to determine whether there is a genetic influence.

In summary, darker iris color was found to be associated with wider retinal vascular caliber, as measured with a standardized semiautomated computer program from digital retinal images. Retinal pigmentation, as approximated by iris color, could be an important source of error in the measurement of retinal vessel caliber, and this measurement error may largely explain the observed ethnic differences in retinal vessel caliber. If this measurement error is confirmed by other studies, the effect of contrast may have to be taken into account when comparing retinal vessel caliber among subjects with different ethnic backgrounds.


   Footnotes
 
Supported by National Health and Medical Research Council, Australia, Grant 253732.

Submitted for publication February 6, 2007; revised April 4, 2007; accepted February 15, 2008.

Disclosure: E. Rochtchina, None; J.J. Wang, None; B. Taylor, None; T.Y. Wong, None; P. Mitchell, None

The publication costs of this article were defrayed in part by page charge payment. This article must therefore be marked "advertisement" in accordance with 18 U.S.C. §1734 solely to indicate this fact.

Corresponding author: Paul Mitchell, Centre for Vision Research, Department of Ophthalmology University of Sydney, Westmead Hospital, Hawkesbury Rd., Westmead, NSW, 2145, Australia; paul_mitchell{at}wmi.usyd.edu.au.


   References
Top
 Abstract
 Methods
 Results
 Discussion
 References
 

  1. Hubbard LD, Brothers RJ, King WN, et al. Methods for evaluation of retinal microvascular abnormalities associated with hypertension/sclerosis in the Atherosclerosis Risk in Communities Study. Ophthalmology. 1999;106:2269–2280.[CrossRef][Web of Science][Medline][Order article via Infotrieve]
  2. Wong TY, Knudtson MD, Klein R, et al. Computer-assisted measurement of retinal vessel diameters in the Beaver Dam Eye Study: methodology, correlation between eyes, and effect of refractive errors. Ophthalmology. 2004;111:1183–1190.[CrossRef][Web of Science][Medline][Order article via Infotrieve]
  3. Knudtson MD, Lee KE, Hubbard LD, et al. Revised formulas for summarizing retinal vessel diameters. Curr Eye Res. 2003;27:143–149.[CrossRef][Web of Science][Medline][Order article via Infotrieve]
  4. Wong TY, Shankar A, Klein R, et al. Prospective cohort study of retinal vessel diameters and risk of hypertension (published correction in BMJ. 2004;329:384). BMJ. 2004;329:79.[Abstract/Free Full Text]
  5. Wong TY, Islam FM, Klein R, et al. Retinal vascular caliber, cardiovascular risk factors, and inflammation: the multi-ethnic study of atherosclerosis (MESA). Invest Ophthalmol Vis Sci. 2006;47:2341–2350.[Abstract/Free Full Text]
  6. Wong TY, Shankar A, Klein R, et al. Retinal arteriolar narrowing, hypertension, and subsequent risk of diabetes mellitus. Arch Intern Med. 2005;165:1060–1065.[Abstract/Free Full Text]
  7. Wong TY, Duncan BB, Golden SH, et al. Associations between the metabolic syndrome and retinal microvascular signs: the Atherosclerosis Risk In Communities study. Invest Ophthalmol Vis Sci. 2004;45:2949–2954.[Abstract/Free Full Text]
  8. Wang JJ, Taylor B, Wong TY, et al. Retinal vessel diameters and obesity: A population-based study in older persons. Obesity (Silver Spring). 2006;14:206–214.[Medline][Order article via Infotrieve]
  9. Smith W, Wang JJ, Wong TY, et al. Retinal arteriolar narrowing is associated with 5-year incident severe hypertension: the Blue Mountains Eye Study. Hypertension. 2004;44:442–447.[Abstract/Free Full Text]
  10. Cheung N, Islam A, Saw SM, et al. Distribution and associations of retinal vascular caliber with ethnicity, gender, and birth parameters in young children. Invest Ophthalmol Vis Sci. 2007;48:1018–1024.[Abstract/Free Full Text]
  11. Wang JJ, Wong TY. Genetic determinants of retinal vascular caliber: additional insights into hypertension pathogenesis. Hypertension. 2006;47:644–645.[Free Full Text]
  12. Xing C, Klein BE, Klein R, et al. Genome-wide linkage study of retinal vessel diameters in the Beaver Dam Eye Study. Hypertension. 2006;47:797–802.[Abstract/Free Full Text]
  13. Sherry LM, Wang JJ, Rochtchina E, et al. Reliability of computer-assisted retinal vessel measurement in a population. Clin Exp Ophthalmol. 2002;30:179–182.[CrossRef][Web of Science][Medline][Order article via Infotrieve]
  14. Ojaimi E, Rose KA, Smith W, et al. Methods for a population-based study of myopia and other eye conditions in school children: the Sydney Myopia Study. Ophthalmic Epidemiol. 2005;12:59–69.[CrossRef][Web of Science][Medline][Order article via Infotrieve]
  15. Ojaimi E, Rose KA, Morgan IG, et al. Distribution of ocular biometric parameters and refraction in a population-based study of Australian children. Invest Ophthalmol Vis Sci. 2005;46:2478–2454.
  16. Hitzenberger CK. Optical measurement of the axial eye length by laser Doppler interferometry. Invest Ophthalmol Vis Sci. 1991;32:616–624.[Abstract/Free Full Text]
  17. Wong TY, Klein R, Sharrett AR, et al. Retinal arteriolar narrowing and risk of coronary heart disease in men and women. The Atherosclerosis Risk in Communities Study. JAMA. 2002;287:1153–1159.[Abstract/Free Full Text]
  18. Mitchell P, Cheung N, de Haseth K, et al. Blood pressure and retinal arteriolar narrowing in children. Hypertension. 2007;49(5)1156–1162.[Abstract/Free Full Text]
  19. Leung H, Wang JJ, Rochtchina E, et al. Computer-assisted retinal vessel measurement in an older population: correlation between right and left eyes. Clin Exp Ophthalmol. 2003;31:326–330.[CrossRef][Web of Science][Medline][Order article via Infotrieve]
  20. Larsson M, Pedersen NL, Stattin H. Importance of genetic effects for characteristics of the human iris. Twin Res. 2003;6:192–200.[CrossRef][Web of Science][Medline][Order article via Infotrieve]




This Article
Right arrow Abstract Freely available
Right arrow Full Text (PDF)
Right arrow Submit a response
Right arrow Alert me when this article is cited
Right arrow Alert me when eLetters are posted
Right arrow Alert me if a correction is posted
Right arrow Citation Map
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in Web of Science
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrow reprints & permissions
Citing Articles
Right arrow Citing Articles via Web of Science (3)
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Rochtchina, E.
Right arrow Articles by Mitchell, P.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Rochtchina, E.
Right arrow Articles by Mitchell, P.

HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS