Abnormal cellular reactivity to microbial antigens in patients with uveitis
Gregoire Jacques Noel COZON 1,
Martial Florentin NBITIKI 1,
Fathi FATOOHI 1,
Myriam SPIRE 2,
Jean-Daniel GRANGE 2,
and
Laurent Kodjikian 3*
1 INSERM U851 Universite Lyon 1, IFR 128, HCL: 69365 Lyon, France. UFR Lyon-Nord Universite Lyon 1 69, Lyon, France; Laboratoire d'Immunologie, Hopital de la Croix-Rousse, Hospices Civils de Lyon, Lyon, France
2 Service d'Ophtalmologie, Hopital de la Croix-Rousse, Hospices Civils de Lyon, Lyon, France
3 Ophthalmology, Croix-Rousse Hospital, EA3090, Lyon, France
* To whom correspondence should be addressed. E-mail: kodjikian.laurent{at}wanadoo.fr.
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Abstract |
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Purpose: The aims of the present study were to evaluate the cellular response to microbial antigens in patients with idiopathic uveitis. Methods: Blood lymphocytes from 31 patients with uveitis and 24 healthy controls were cultivated with microbial antigens and analyzed in flow cytometry after staining with monoclonal antibodies against CD3, CD4, and activation markers CD69 and CD25. Results: Although no difference was noted in circulating lymphocytes, activation of T-cells, detected with CD69, was higher in a 24-hour blood culture from uveitis patients with Candida albicans antigen (Ca-Ag) when compared to controls, especially in posterior uveitis and panuveitis. Moreover late response, detected with CD25, to different microbial antigens was higher in patient with uveitis. Conclusions: Such results suggest the role of Ca-Ag and microbial antigens in the pathogenic mechanisms of idiopathic uveitis.
Key Words:
uveitis, immunocytochemistry, microbial antigen, candida, cellular immunity
