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A more recent version of this article appeared on August 1, 2008
 (Investigative Ophthalmology and Visual Science. )
© 2008 by The Association for Research in Vision and Ophthalmology, Inc.
doi:10.1167/iovs.08-1679
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Article

Early inner retinal dysfunction in streptozotocin induced diabetic rats

Kenichi Kozaki 1, Algis J. Vingrys 2, and Bang Viet Bui 3*

1 Department of Ophthalmology, The Jikei University, Tokyo, Nishi-shimbashi, Japan
2 Optometry and Vision Sciences, The University of Melbourne, Melbourne, Victoria, Australia
3 Optometry and Vision Sciences, The University of Melbourne, Corner, Cardigan & Kepple Streets, Melbourne, Victoria, 3010, Australia

* To whom correspondence should be addressed. E-mail: bvb{at}unimelb.edu.au.


  Abstract

PURPOSE. Diabetes is known to alter retinal function as measured with the electroretinogram (ERG) where a propensity is found for inner-retinal, oscillatory potential (OPs) abnormalities. However the effect that diabetes has on other ganglion cell-related responses is not known. This study reports a systematic evaluation of streptozotocin (STZ) diabetes related ERG changes in rats for the first 11 weeks post-diabetogenesis. METHODS. Thirty, Sprague-Dawley rats were randomly assigned to treated (50 mg/kg STZ (n = 16) and control groups (1 mL/kg citrate buffer, n = 14) at 6 weeks of age. Two controls and 4 STZ animals were excluded due to blood glucose criteria or systemic complications. Diabetic animals were given daily subcutaneous injections of 1-2 units of long-acting insulin. ERGs were measured at 4, 8 and 11 weeks after treatment. The a-wave was used as an index of outer-retinal function whereas b-wave, OPs and the scotopic threshold response (STR) were used as indices of inner retinal function. RESULTS. Photoreceptoral (a-wave) and bipolar cell (b-wave) responses were not significantly reduced by STZ treatment. OPs were significantly reduced by 8 weeks (-25 ± 7%, P < 0.05). The most severely affected component was the ganglion cell dominated positive STR, which was significantly decreased from the first time-point (-51 ± 11% at 4 weeks, P < 0.05), but the negative component was unaffected over the 11 week period. CONCLUSIONS. The ganglion cell dominated pSTR showed large losses in STZ treated rats.

Key Words: diabetic retinopathy, electroretinography, ganglion cell




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N. P. Blair, J. M. Wanek, M. Mori, and M. Shahidi
Abnormal Retinal Vascular Oxygen Tension Response to Light Flicker in Diabetic Rats
Invest. Ophthalmol. Vis. Sci., November 1, 2009; 50(11): 5444 - 5448.
[Abstract] [Full Text] [PDF]


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